Recombinant Rhipicephalus sanguineus Evasin-3, Rhipicephalus sanguineus (Brown dog tick) (Ixodes sanguineus), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 14.4kDa, purity >85% as determined by SDS-PAGE.

Recombinant Rhipicephalus sanguineus Evasin-3

Recombinant Rhipicephalus sanguineus Evasin-3 — Salivary chemokine-binding protein which shows chemokine neutralizing activity and binds to host chemokines CXCL1, CXCL2, CXCL3, CXCL5, CXCL6 and CXCL8. Purity >85%.

SKU: BCRECP-003574 Category:
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Product Specifications

Product SkuBCRECP-003574
Product DescriptionRecombinant Rhipicephalus sanguineus Evasin-3 Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 21-86aa. [Accession | P0C8E8].
Uniprot No.P0C8E8
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region21-86aa
SpeciesRhipicephalus sanguineus (Brown dog tick) (Ixodes sanguineus)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight14.4kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Salivary chemokine-binding protein which shows chemokine neutralizing activity and binds to host chemokines CXCL1, CXCL2, CXCL3, CXCL5, CXCL6 and CXCL8. Binds to CXCL8 with 1:1 stoichiometry. Disrupts CXCL8 homodimer formation, disrupts the glycosaminoglycan-binding site of CXCL8 and inhibits the interaction of CXCL8 with CXCR2.

Biological Context

Subcellular Location: Secreted

Product Specifications

Recombinant Rhipicephalus sanguineus Evasin-3 is a recombinant protein from Rhipicephalus sanguineus (Brown dog tick) (Ixodes sanguineus), expressed in E.coli, covering amino acids 21-86aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 14.4kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Salivary chemokine-binding protein which shows chemokine neutralizing activity and binds to host chemokines CXCL1, CXCL2, CXCL3, CXCL5, CXCL6 and CXCL8 (PubMed:18678732, PubMed:31167786, PubMed:31235521). Binds to CXCL8 with 1:1 stoichiometry (PubMed:31235521). Disrupts CXCL8 homodimer formation, disrupts the glycosaminoglycan-binding site of CXCL8 and inhibits the interaction of CXCL8 with CXCR2 (PubMed:31235521)

Subcellular Location

Secreted

Subunit

Monomer

Organism: Rhipicephalus sanguineus

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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