Recombinant Conus striatus Con-ikot-ikot, Conus striatus (Striated cone), E.coli, N-terminal 6xHis-tagged, 13.4kDa, purity >90% as determined by SDS-PAGE.

Recombinant Conus striatus Con-ikot-ikot

Recombinant Conus striatus Con-ikot-ikot — Potently and selectively blocks the desensitization of ionotropic glutamate AMPA receptors (GRIA1, GRIA2, GRIA3 and GRIA4). Purity >90%.

SKU: BCRECP-003579 Category:
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Product Specifications

Product SkuBCRECP-003579
Product DescriptionRecombinant Conus striatus Con-ikot-ikot Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 38-123aa. [Accession | P0CB20].
Uniprot No.P0CB20
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region38-123aa
SpeciesConus striatus (Striated cone)
Tag InfoN-terminal 6xHis-tagged
Molecular weight13.4kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Potently and selectively blocks the desensitization of ionotropic glutamate AMPA receptors (GRIA1, GRIA2, GRIA3 and GRIA4). Binds to a different site than does the drug cyclothiazide. The toxin acts like a straitjacket on the 'gating ring' of the ligand-binding domain (LBD) of the receptor.

Biological Context

Subcellular Location: Secreted
Tissue Specificity: Expressed by the venom duct

Product Specifications

Recombinant Conus striatus Con-ikot-ikot is a recombinant protein from Conus striatus (Striated cone), expressed in E.coli, covering amino acids 38-123aa, with N-terminal 6xHis-tagged tag, molecular weight 13.4kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Potently and selectively blocks the desensitization of ionotropic glutamate AMPA receptors (GRIA1, GRIA2, GRIA3 and GRIA4) (PubMed:19481459, PubMed:35377397). Binds to a different site than does the drug cyclothiazide (PubMed:19481459). The toxin acts like a straitjacket on the 'gating ring' of the ligand-binding domain (LBD) of the receptor (PubMed:25103405). It does so by restraining the domains via both intra- and interdimer cross-links such that agonist-induced closure of the LBD 'clamshells' is transduced into an irislike expansion of the gating ring (PubMed:25103405). Compared to other desensitization blockers, it is a poor stabilizer of the open channel because toxin-bound AMPA receptors undergo frequent brief closures (PubMed:35377397). In vitro, application of the toxin to hippocampal slices causes a large and rapid increase in resting AMPA receptor-mediated current leading to neuronal death (PubMed:19481459)

Subcellular Location

Secreted

Tissue Specificity

Expressed by the venom duct

Subunit

Homodimer; disulfide-linked

Organism: Conus striatus

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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