Recombinant Kukulcania hibernalis Omega-filistatoxin-Kh1a, Kukulcania hibernalis (Southern house spider) (Filistata hibernalis), E.coli, C-terminal 6xHis-tagged, 15.6kDa, purity >95% as determined by SDS-PAGE.

Recombinant Kukulcania hibernalis Omega-filistatoxin-Kh1a

Recombinant Kukulcania hibernalis Omega-filistatoxin-Kh1a — Potently blocks vertebrate calcium channels Cav1 and Cav2. Purity >95%.

SKU: BCRECP-003652 Category:
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Product Specifications

Product SkuBCRECP-003652
Product DescriptionRecombinant Kukulcania hibernalis Omega-filistatoxin-Kh1a Protein is expressed from E.coli with C-terminal 6xHis-tagged. It contains 1-74aa. [Accession | P60979].
Uniprot No.P60979
PurityGreater than 95% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-74aa
SpeciesKukulcania hibernalis (Southern house spider) (Filistata hibernalis)
Tag InfoC-terminal 6xHis-tagged
Molecular weight15.6kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Potently blocks vertebrate calcium channels Cav1 and Cav2. Is the most active on Cav2.2/CACNA1B (from HEK) (IC(50)=2.3 nM), followed by Cav2.1/CACNA1A (IC(50)=4.3 nM), Cav2.2/CACNA1B (from oocyte) (IC(50)=14.4 nM), Cav1.2/CACNA1C (IC(50)=26.8 nM), and Cav2.3/CACNA1E (IC(50)=96.4 nM).

Product Specifications

Recombinant Kukulcania hibernalis Omega-filistatoxin-Kh1a is a recombinant protein, expressed in E.coli, covering amino acids 1-74aa, with C-terminal 6xHis-tagged tag, molecular weight 15.6kDa, purity Greater than 95% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Potently blocks vertebrate calcium channels Cav1 and Cav2. Is the most active on Cav2.2/CACNA1B (from HEK) (IC(50)=2.3 nM), followed by Cav2.1/CACNA1A (IC(50)=4.3 nM), Cav2.2/CACNA1B (from oocyte) (IC(50)=14.4 nM), Cav1.2/CACNA1C (IC(50)=26.8 nM), and Cav2.3/CACNA1E (IC(50)=96.4 nM)

Organism: Kukulcania hibernalis

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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