Recombinant Putative N-acetylmannosamine-6-phosphate 2-epimerase (nanE), Clostridium perfringens (strain 13 / Type A), Yeast, C-terminal 6xHis-tagged, 25.7kDa, purity >85% as determined by SDS-PAGE.

Recombinant Putative N-acetylmannosamine-6-phosphate 2-epimerase (nanE)

Recombinant Putative N-acetylmannosamine-6-phosphate 2-epimerase (nanE) — Epimerase involved in the degradation of sialic acid. Purity >85%.

SKU: BCRECP-003848 Category:
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Product Specifications

Product SkuBCRECP-003848
Product DescriptionRecombinant Putative N-acetylmannosamine-6-phosphate 2-epimerase (nanE) Protein is expressed from Yeast with C-terminal 6xHis-tagged. It contains 1-221aa. [Accession | Q8XNZ3].
Uniprot No.Q8XNZ3
Gene NamesnanE
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemYeast
Expression Region1-221aa
SpeciesClostridium perfringens (strain 13 / Type A)
Tag InfoC-terminal 6xHis-tagged
Molecular weight25.7kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Epimerase involved in the degradation of sialic acid. Converts N-acetylmannosamine-6-phosphate (ManNAc-6-P) to N-acetylglucosamine-6-phosphate (GlcNAc-6-P).

Biological Context

Pathway: Amino-sugar metabolism; N-acetylneuraminate degradation; D-fructose 6-phosphate from N-acetylneuraminate: step 3/5

Product Specifications

Recombinant Putative N-acetylmannosamine-6-phosphate 2-epimerase (nanE) is a recombinant protein from Clostridium perfringens (strain 13 / Type A), expressed in Yeast, covering amino acids 1-221aa, with C-terminal 6xHis-tagged tag, molecular weight 25.7kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Epimerase involved in the degradation of sialic acid (PubMed:25320079). Converts N-acetylmannosamine-6-phosphate (ManNAc-6-P) to N-acetylglucosamine-6-phosphate (GlcNAc-6-P) (PubMed:25320079)

Subunit

Homodimer

Pathway

Amino-sugar metabolism; N-acetylneuraminate degradation; D-fructose 6-phosphate from N-acetylneuraminate: step 3/5

Gene: nanE  |  Organism: Clostridium perfringens (strain 13 / Type A)  |  Synonyms: ManNAc-6-P epimerase; ManNAc-6P 2-epimerase

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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