Recombinant Mouse Homeobox protein Nkx-3.2 (Nkx3-2), Mus musculus (Mouse), E.coli, N-terminal 10xHis-tagged, 35.2kDa, purity >85% as determined by SDS-PAGE.

Recombinant Mouse Homeobox protein Nkx-3.2 (Nkx3-2)

Recombinant Mouse Homeobox protein Nkx-3.2 (Nkx3-2) — Transcriptional repressor that acts as a negative regulator of chondrocyte maturation. Purity >85%.

SKU: BCRECP-003881 Category:
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Product Specifications

Product SkuBCRECP-003881
Product DescriptionRecombinant Mouse Homeobox protein Nkx-3.2 (Nkx3-2) Protein is expressed from E.coli with N-terminal 10xHis-tagged. It contains 1-333aa. [Accession | P97503].
Uniprot No.P97503
Gene NamesNkx3-2
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-333aa
SpeciesMus musculus (Mouse)
Tag InfoN-terminal 10xHis-tagged
Molecular weight35.2kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasEpigenetics and Nuclear Signaling

Function

Transcriptional repressor that acts as a negative regulator of chondrocyte maturation. PLays a role in distal stomach development; required for proper antral-pyloric morphogenesis and development of antral-type epithelium. In concert with GSC, defines the structural components of the middle ear; required for tympanic ring and gonium development and in the regulation of the width of the malleus.

Biological Context

Subcellular Location: Nucleus
Tissue Specificity: Expressed widely in mesoderm at the gastroduodenal junction (at protein level). Expressed in visceral mesoderm and embryonic skeleton. Expression is restricted to immature proliferative chondrocytes during endochondral ossification

Product Specifications

Recombinant Mouse Homeobox protein Nkx-3.2 (Nkx3-2) is a recombinant protein from Mus musculus (Mouse), expressed in E.coli, covering amino acids 1-333aa, with N-terminal 10xHis-tagged tag, molecular weight 35.2kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Transcriptional repressor that acts as a negative regulator of chondrocyte maturation. PLays a role in distal stomach development; required for proper antral-pyloric morphogenesis and development of antral-type epithelium. In concert with GSC, defines the structural components of the middle ear; required for tympanic ring and gonium development and in the regulation of the width of the malleus

Subcellular Location

Nucleus

Tissue Specificity

Expressed widely in mesoderm at the gastroduodenal junction (at protein level). Expressed in visceral mesoderm and embryonic skeleton. Expression is restricted to immature proliferative chondrocytes during endochondral ossification

Gene: Nkx3-2  |  Organism: Mus musculus  |  Synonyms: Bagpipe homeobox protein homolog 1; Homeobox protein NK-3 homolog B
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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