Recombinant Hepatitis E virus genotype 3 Pro-secreted protein ORF2 (ORF2), partial, Hepatitis E virus genotype 3 (isolate Human/United States/US2) (HEV-3), E.coli, N-terminal 6xHis-tagged, 31.4kDa, purity >85% as determined by SDS-PAGE.
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Recombinant Hepatitis E virus genotype 3 Pro-secreted protein ORF2 (ORF2), partial

Recombinant Hepatitis E virus genotype 3 Pro-secreted protein ORF2 (ORF2), partial — Forms an icosahedral capsid with a T=1 symmetry and a 34 nm diameter. Purity >85%.

SKU: BCRECP-003969 Categories: , ,
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Product Specifications

Product SkuBCRECP-003969
Product DescriptionRecombinant Hepatitis E virus genotype 3 Pro-secreted protein ORF2 (ORF2) Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 411-660aa. [Accession | Q9YLQ9].
Uniprot No.Q9YLQ9
Gene NamesORF2
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region411-660aa
SpeciesHepatitis E virus genotype 3 (isolate Human/United States/US2) (HEV-3)
Tag InfoN-terminal 6xHis-tagged
Molecular weight31.4kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasMicrobiology

Function

Forms an icosahedral capsid with a T=1 symmetry and a 34 nm diameter. The capsid is composed of 60 copies linked to each other. Binds to the 5' end of the genomic RNA to mediate genome encapsidation.

Biological Context

Subcellular Location: Virion; Host cytoplasm; Host endoplasmic reticulum; Host Golgi apparatus; Host cell surface; Host nucleus

Product Specifications

Recombinant Hepatitis E virus genotype 3 Pro-secreted protein ORF2 (ORF2), partial is a recombinant protein from Hepatitis E virus genotype 3 (isolate Human/United States/US2) (HEV-3), expressed in E.coli, covering amino acids 411-660aa, with N-terminal 6xHis-tagged tag, molecular weight 31.4kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Forms an icosahedral capsid with a T=1 symmetry and a 34 nm diameter. The capsid is composed of 60 copies linked to each other. Binds to the 5' end of the genomic RNA to mediate genome encapsidation (By similarity). Binds to heparin surface proteoglycans (HSPGs) to mediate viral entry. Additionally, the interactions with host ASGR1 and ASGR2 facilitate viral infection of hepatocytes (By similarity). Inhibits IFN production by blocking host TBK1-induced IRF3 phosphorylation (By similarity). The nuclear form probably modulates host gene expression (By similarity)

Subcellular Location

Virion; Host cytoplasm; Host endoplasmic reticulum; Host Golgi apparatus; Host cell surface; Host nucleus

Subunit

Self-assembles to form the capsid. The capsid is dominated by dimers that define the 30 morphological units. Interacts with phosphorylated protein ORF3 (By similarity). Interacts with host TMEM134. Interacts with host ASGR1 and ASGR2; these interactions facilitate infection of host hepatocytes (By similarity)

Organism: Hepatitis E virus genotype 3 (isolate Human/United States/US2)  |  Synonyms: Protein ORF2

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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