Recombinant Hepatitis E virus genotype 1 Protein ORF3 (ORF3), Hepatitis E virus genotype 1 (isolate Human/Pakistan/Sar-55) (HEV-1), E.coli, N-terminal 6xHis-SUMO-tagged, 27.8kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Hepatitis E virus genotype 1 Protein ORF3 (ORF3)

Recombinant Hepatitis E virus genotype 1 Protein ORF3 (ORF3) — Small multifunctional phosphoprotein involved in virion morphogenesis, egress and counteracting host innate immunity. Purity >90%.

SKU: BCRECP-003971 Categories: , ,
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Product Specifications

Product SkuBCRECP-003971
Product DescriptionRecombinant Hepatitis E virus genotype 1 Protein ORF3 (ORF3) Protein is expressed from E.coli with N-terminal 6xHis-SUMO-tagged. It contains 1-114aa. [Accession | O90299].
Uniprot No.O90299
Gene NamesORF3
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-114aa
SpeciesHepatitis E virus genotype 1 (isolate Human/Pakistan/Sar-55) (HEV-1)
Tag InfoN-terminal 6xHis-SUMO-tagged
Molecular weight27.8kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Small multifunctional phosphoprotein involved in virion morphogenesis, egress and counteracting host innate immunity. Plays critical roles in the final steps of viral release by interacting with host TSG101, a member of the vacuolar protein-sorting pathway and using other cellular host proteins involved in vesicle formation pathway. Also acts as a viroporin and forms ion conductive pores allowing viral particle release.

Biological Context

Subcellular Location: Host endoplasmic reticulum membrane (Lipid-anchor); Host cytoplasm, host cytoskeleton; Virion; Host cell membrane (Lipid-anchor)

Product Specifications

Recombinant Hepatitis E virus genotype 1 Protein ORF3 (ORF3) is a recombinant protein from Hepatitis E virus genotype 1 (isolate Human/Pakistan/Sar-55) (HEV-1), expressed in E.coli, covering amino acids 1-114aa, with N-terminal 6xHis-SUMO-tagged tag, molecular weight 27.8kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Small multifunctional phosphoprotein involved in virion morphogenesis, egress and counteracting host innate immunity. Plays critical roles in the final steps of viral release by interacting with host TSG101, a member of the vacuolar protein-sorting pathway and using other cellular host proteins involved in vesicle formation pathway. Also acts as a viroporin and forms ion conductive pores allowing viral particle release. Impairs the generation of type I interferon by down-regulating host TLR3 and TLR7 as well as their downstream signaling pathways. Down-regulates the phosphorylation of host IRF3 via the interaction with host SIRP-alpha, thereby inhibiting IFN-I expression. Interacts with host microtubules

Subcellular Location

Host endoplasmic reticulum membrane (Lipid-anchor); Host cytoplasm, host cytoskeleton; Virion; Host cell membrane (Lipid-anchor)

Subunit

Forms homooligomers (By similarity). Interacts with host SRC, HCK, FYN, PIK3R3 and GRB2 (via SH3 domain); binding does not activate the kinases. Interacts with host AMBP/bikunin and AMBP/alpha-1-microglobulin peptides. Interacts with host HPX/hemopexin. Interacts (when phosphorylated) with capsid protein ORF2 (PubMed:11160756, PubMed:11518702, PubMed:11934888, PubMed:15037615, PubMed:16140784, PubMed:18211098). Interacts with host TSG101; this interaction plays a role in viral release from the host cell (By similarity). Interacts with host SIRPA; this interaction down-regulates the phosphorylation of host IRF3 (By similarity)

Organism: Hepatitis E virus genotype 1 (isolate Human/India/Hyderabad)
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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