Recombinant Escherichia coli Peptidoglycan-associated lipoprotein (pal), Escherichia coli (strain K12), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 24.1kDa, purity >85% as determined by SDS-PAGE.

Recombinant Escherichia coli Peptidoglycan-associated lipoprotein (pal)

Recombinant Escherichia coli Peptidoglycan-associated lipoprotein (pal) — Part of the Tol-Pal system, which plays a role in outer membrane invagination during cell division and is important for maintaining outer membrane integrity. Purity >85%.

SKU: BCRECP-004019 Category:
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Product Specifications

Product SkuBCRECP-004019
Product DescriptionRecombinant Escherichia coli Peptidoglycan-associated lipoprotein (pal) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 22-173aa. [Accession | P0A912].
Uniprot No.P0A912
Gene Namespal
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region22-173aa
SpeciesEscherichia coli (strain K12)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight24.1kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasMicrobiology

Function

Part of the Tol-Pal system, which plays a role in outer membrane invagination during cell division and is important for maintaining outer membrane integrity. The Tol-Pal system is also required for polar localization of chemoreceptors clusters. The system also appears to be required for the activity of several outer membrane-localized enzymes with cell wall remodeling activity.

Biological Context

Subcellular Location: Cell outer membrane (Lipid-anchor)

Product Specifications

Recombinant Escherichia coli Peptidoglycan-associated lipoprotein (pal) is a recombinant protein from Escherichia coli (strain K12), expressed in E.coli, covering amino acids 22-173aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 24.1kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Part of the Tol-Pal system, which plays a role in outer membrane invagination during cell division and is important for maintaining outer membrane integrity (PubMed:11115123, PubMed:17233825). The Tol-Pal system is also required for polar localization of chemoreceptors clusters (PubMed:24720726). The system also appears to be required for the activity of several outer membrane-localized enzymes with cell wall remodeling activity (PubMed:32152098)

Subcellular Location

Cell outer membrane (Lipid-anchor)

Subunit

The Tol-Pal system is composed of five core proteins: the inner membrane proteins TolA, TolQ and TolR, the periplasmic protein TolB and the outer membrane protein Pal. They form a network linking the inner and outer membranes and the peptidoglycan layer (PubMed:17233825). Pal interacts with TolB (PubMed:17375930, PubMed:19696740, PubMed:7744736). Interacts with the C-terminal domain of TolA. This interaction is proton motive force dependent and requires TolQ and TolR (PubMed:11115123, PubMed:11722743). Can form a complex with TolB and OmpA (PubMed:9701827)

Gene: pal  |  Organism: Escherichia coli (strain K12)  |  Synonyms: Tol-Pal system lipoprotein Pal
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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