Recombinant Sheep Platelet-derived growth factor subunit B (PDGFB), Ovis aries (Sheep), E.coli, N-terminal 6xHis-TrxA-tagged, 30.6kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Sheep Platelet-derived growth factor subunit B (PDGFB)

Recombinant Sheep Platelet-derived growth factor subunit B (PDGFB) — Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Purity >90%.

SKU: BCRECP-004040 Categories: ,
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Product Specifications

Product SkuBCRECP-004040
Product DescriptionRecombinant Sheep Platelet-derived growth factor subunit B (PDGFB) Protein is expressed from E.coli with N-terminal 6xHis-TrxA-tagged. It contains 82-190aa. [Accession | Q95229].
Uniprot No.Q95229
Gene NamesPDGFB
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region82-190aa
SpeciesOvis aries (Sheep)
Tag InfoN-terminal 6xHis-TrxA-tagged
Molecular weight30.6kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasCancer

Function

Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta.

Biological Context

Subcellular Location: Secreted

Product Specifications

Recombinant Sheep Platelet-derived growth factor subunit B (PDGFB) is a recombinant protein from Ovis aries (Sheep), expressed in E.coli, covering amino acids 82-190aa, with N-terminal 6xHis-TrxA-tagged tag, molecular weight 30.6kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. Required for normal blood vessel development, and for normal development of kidney glomeruli. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFA (By similarity)

Subcellular Location

Secreted

Subunit

Antiparallel homodimer; disulfide-linked. Antiparallel heterodimer with PDGFA; disulfide-linked. The PDGFB homodimer interacts with PDGFRA and PDGFRB homodimers, and with heterodimers formed by PDGFRA and PDGFRB. The heterodimer composed of PDGFA and PDGFB interacts with PDGFRB homodimers, and with heterodimers formed by PDGFRA and PDGFRB. Interacts with XLKD1 (By similarity). Interacts with LRP1. Interacts with SORL1 (via the N-terminal ectodomain). Interacts with CD82; this interaction inhibits PDGFB-mediated signaling pathway (By similarity)

Gene: PDGFB  |  Organism: Ovis aries  |  Synonyms: PDGF-2; Platelet-derived growth factor B chain; Platelet-derived growth factor beta polypeptide

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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