Recombinant Pseudomonas aeruginosa Type IV pilus biogenesis factor PilY1 (pilY1), partial, Pseudomonas aeruginosa (strain PAK), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 46.2kDa, purity >90% as determined by SDS-PAGE.

Recombinant Pseudomonas aeruginosa Type IV pilus biogenesis factor PilY1 (pilY1), partial

Recombinant Pseudomonas aeruginosa Type IV pilus biogenesis factor PilY1 (pilY1), partial — Involved in pilus assembly, twitching motility and adhesion to host cells. Purity >90%.

SKU: BCRECP-004080 Category:
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Product Specifications

Product SkuBCRECP-004080
Product DescriptionRecombinant Pseudomonas aeruginosa Type IV pilus biogenesis factor PilY1 (pilY1) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 610-970aa. [Accession | S0HPF7].
Uniprot No.S0HPF7
Gene NamespilY1
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region610-970aa
SpeciesPseudomonas aeruginosa (strain PAK)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight46.2kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Involved in pilus assembly, twitching motility and adhesion to host cells. Primes type IV pili (T4P) assembly and is required for inclusion of minor pilins PilV, PilW and PilX to the surface pili. Stabilizes assembled pilus fibers likely by antagonizing retraction mediated by PilT.

Biological Context

Subcellular Location: Fimbrium; Membrane; Cytoplasm, cytosol

Product Specifications

Recombinant Pseudomonas aeruginosa Type IV pilus biogenesis factor PilY1 (pilY1), partial is a recombinant protein from Pseudomonas aeruginosa (strain PAK), expressed in E.coli, covering amino acids 610-970aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 46.2kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Involved in pilus assembly, twitching motility and adhesion to host cells. Primes type IV pili (T4P) assembly and is required for inclusion of minor pilins PilV, PilW and PilX to the surface pili (By similarity). Stabilizes assembled pilus fibers likely by antagonizing retraction mediated by PilT. Calcium-binding and calcium release by PilY1 seem to be essential for twitching motility and for regulation of pilus retraction dynamics of PilT. Adhesin for human tissue specifically recognizing a host receptor localized or enriched on basolateral epithelial cell surfaces. Binds host integrins in an calcium-dependent manner in vitro and this interaction may be employed by the bacterium to mediate host epithelial cell binding in vivo

Subcellular Location

Fimbrium; Membrane; Cytoplasm, cytosol

Subunit

Interacts (via C-terminal 532-1163) with host integrins alpha-V/beta-3 (ITGAV/ITGB3) and alpha-V/beta-5 (ITGAV/ITGB5)

Gene: pilY1  |  Organism: Pseudomonas aeruginosa (strain PAK)  |  Synonyms: Pilus-associated adhesin PilY
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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