Recombinant Streptococcus pneumoniae serotype 4 Pneumolysin (ply), Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4), E.coli, N-terminal 6xHis-Avi-tagged, 58.7kDa, purity >85% as determined by SDS-PAGE.

Recombinant Streptococcus pneumoniae serotype 4 Pneumolysin (ply)

Recombinant Streptococcus pneumoniae serotype 4 Pneumolysin (ply) — A cholesterol-dependent toxin that causes cytolysis by forming pores in cholesterol containing host membranes (Probable). Purity >85%.

SKU: BCRECP-004108 Category:
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Product Specifications

Product SkuBCRECP-004108
Product DescriptionRecombinant Streptococcus pneumoniae serotype 4 Pneumolysin (ply) Protein is expressed from E.coli with N-terminal 6xHis-Avi-tagged. It contains 2-471aa. [Accession | P0C2J9].
Uniprot No.P0C2J9
Gene Namesply
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region2-471aa
SpeciesStreptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
Tag InfoN-terminal 6xHis-Avi-tagged
Molecular weight58.7kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

A cholesterol-dependent toxin that causes cytolysis by forming pores in cholesterol containing host membranes (Probable). After binding to target membranes, the protein undergoes a major conformation change, leading to its insertion in the host membrane and formation of an oligomeric pore complex. Cholesterol is required for binding to host membranes, membrane insertion and pore formation; cholesterol binding is mediated by a Thr-Leu pair in the C-terminus.

Biological Context

Subcellular Location: Secreted; Secreted, cell wall; Host cell membrane (Multi-pass membrane protein)

Product Specifications

Recombinant Streptococcus pneumoniae serotype 4 Pneumolysin (ply) is a recombinant protein from Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4), expressed in E.coli, covering amino acids 2-471aa, with N-terminal 6xHis-Avi-tagged tag, molecular weight 58.7kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

A cholesterol-dependent toxin that causes cytolysis by forming pores in cholesterol containing host membranes (Probable). After binding to target membranes, the protein undergoes a major conformation change, leading to its insertion in the host membrane and formation of an oligomeric pore complex. Cholesterol is required for binding to host membranes, membrane insertion and pore formation; cholesterol binding is mediated by a Thr-Leu pair in the C-terminus. Can be reversibly inactivated by oxidation

Subcellular Location

Secreted; Secreted, cell wall; Host cell membrane (Multi-pass membrane protein)

Subunit

Homooligomeric pore complex of 35 to 50 subunits; when inserted in the host membrane

Gene: ply  |  Organism: Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)  |  Synonyms: Thiol-activated cytolysin

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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