Recombinant Rat Purine nucleoside phosphorylase (Pnp), Rattus norvegicus (Rat), Baculovirus, C-terminal 6xHis-tagged, 33.1kDa, purity >90% as determined by SDS-PAGE.

Recombinant Rat Purine nucleoside phosphorylase (Pnp)

Recombinant Rat Purine nucleoside phosphorylase (Pnp) — Catalyzes the phosphorolytic breakdown of the N-glycosidic bond in the beta-(deoxy)ribonucleoside molecules, with the formation of the corresponding free purine bases and pentose-1-phosphate. Purity >90%.

SKU: BCRECP-004131 Category:
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Product Specifications

Product SkuBCRECP-004131
Product DescriptionRecombinant Rat Purine nucleoside phosphorylase (Pnp) Protein is expressed from Baculovirus with C-terminal 6xHis-tagged. It contains 1-289aa. [Accession | P85973].
Uniprot No.P85973
Gene NamesPnp
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemBaculovirus
Expression Region1-289aa
SpeciesRattus norvegicus (Rat)
Tag InfoC-terminal 6xHis-tagged
Molecular weight33.1kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Catalyzes the phosphorolytic breakdown of the N-glycosidic bond in the beta-(deoxy)ribonucleoside molecules, with the formation of the corresponding free purine bases and pentose-1-phosphate. Preferentially acts on 6-oxopurine nucleosides including inosine and guanosine.

Biological Context

Subcellular Location: Cytoplasm, cytosol
Pathway: Purine metabolism; purine nucleoside salvage

Product Specifications

Recombinant Rat Purine nucleoside phosphorylase (Pnp) is a recombinant protein from Rattus norvegicus (Rat), expressed in Baculovirus, covering amino acids 1-289aa, with C-terminal 6xHis-tagged tag, molecular weight 33.1kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Catalyzes the phosphorolytic breakdown of the N-glycosidic bond in the beta-(deoxy)ribonucleoside molecules, with the formation of the corresponding free purine bases and pentose-1-phosphate (By similarity). Preferentially acts on 6-oxopurine nucleosides including inosine and guanosine (By similarity)

Subcellular Location

Cytoplasm, cytosol

Subunit

Homotrimer

Pathway

Purine metabolism; purine nucleoside salvage

Gene: Pnp  |  Organism: Rattus norvegicus  |  Synonyms: Inosine phosphorylase; Inosine-guanosine phosphorylase

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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