Recombinant Mouse Palmitoyl-protein thioesterase 1 (Ppt1), Mus musculus (Mouse), Mammalian cell, C-terminal 10xHis-tagged, 33.0kDa, purity >90% as determined by SDS-PAGE.
,

Recombinant Mouse Palmitoyl-protein thioesterase 1 (Ppt1)

Recombinant Mouse Palmitoyl-protein thioesterase 1 (Ppt1) — Has thioesterase activity against fatty acid thioesters with 14 -18 carbons, including palmitoyl-CoA, S-palmitoyl-N-acetylcysteamine, and palmitoylated proteins. Purity >90%.

SKU: BCRECP-004147 Categories: ,
Online Inquiry →

Product Specifications

Product SkuBCRECP-004147
Product DescriptionRecombinant Mouse Palmitoyl-protein thioesterase 1 (Ppt1) Protein is expressed from Mammalian cell with C-terminal 10xHis-tagged. It contains 28-306aa. [Accession | O88531].
Uniprot No.O88531
Gene NamesPpt1
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemMammalian cell
Expression Region28-306aa
SpeciesMus musculus (Mouse)
Tag InfoC-terminal 10xHis-tagged
Molecular weight33.0kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Has thioesterase activity against fatty acid thioesters with 14 -18 carbons, including palmitoyl-CoA, S-palmitoyl-N-acetylcysteamine, and palmitoylated proteins. In contrast to PPT2, PPT1 can hydrolyze palmitoylated proteins and palmitoylcysteine.

Biological Context

Subcellular Location: Lysosome; Secreted; Golgi apparatus; Endoplasmic reticulum
Tissue Specificity: Highest level in testis and kidney, lower in heart, brain and lung and lowest in skeletal muscle

Product Specifications

Recombinant Mouse Palmitoyl-protein thioesterase 1 (Ppt1) is a recombinant protein from Mus musculus (Mouse), expressed in Mammalian cell, covering amino acids 28-306aa, with C-terminal 10xHis-tagged tag, molecular weight 33.0kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Has thioesterase activity against fatty acid thioesters with 14 -18 carbons, including palmitoyl-CoA, S-palmitoyl-N-acetylcysteamine, and palmitoylated proteins. In contrast to PPT2, PPT1 can hydrolyze palmitoylated proteins and palmitoylcysteine

Subcellular Location

Lysosome; Secreted; Golgi apparatus; Endoplasmic reticulum

Tissue Specificity

Highest level in testis and kidney, lower in heart, brain and lung and lowest in skeletal muscle

Subunit

Interacts with CLN5, ATP5F1A and ATP5F1B (PubMed:19941651)

Gene: Ppt1  |  Organism: Mus musculus  |  Synonyms: Palmitoyl-protein hydrolase 1
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

Shopping Cart
Scroll to Top