Recombinant Mouse Prominin-1 (Prom1), partial, Mus musculus (Mouse), E.coli, N-terminal 6XHis-SUMO-tagged, 48.5kDa, purity >90% as determined by SDS-PAGE.

Recombinant Mouse Prominin-1 (Prom1), partial

Recombinant Mouse Prominin-1 (Prom1), partial — May play a role in cell differentiation, proliferation and apoptosis. Purity >90%.

SKU: BCRECP-004173 Category:
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Product Specifications

Product SkuBCRECP-004173
Product DescriptionRecombinant Mouse Prominin-1 (Prom1) Protein is expressed from E.coli with N-terminal 6XHis-SUMO-tagged. It contains 509-794aa. [Accession | O54990].
Uniprot No.O54990
Gene NamesProm1
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region509-794aa
SpeciesMus musculus (Mouse)
Tag InfoN-terminal 6XHis-SUMO-tagged
Molecular weight48.5kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

May play a role in cell differentiation, proliferation and apoptosis. Binds cholesterol in cholesterol-containing plasma membrane microdomains and may play a role in the organization of the apical plasma membrane in epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis.

Biological Context

Subcellular Location: Apical cell membrane (Multi-pass membrane protein); Cell projection, microvillus membrane (Multi-pass membrane protein); Cell projection, cilium, photoreceptor outer segment; Endoplasmic reticulum; Endoplasmic reticulum-Golgi intermediate compartment
Tissue Specificity: In the submandibular gland, expressed on the apical side of epithelial cells. In the parotid gland, expressed in the intercalated ducts. In the sublingual gland, expressed in intercalated ducts. In the extraorbital lacrimal gland, expressed in the intercalated tubules and larger intralobular ducts. Expressed in the retina. Present in urine within small membrane particles (at protein level). In the embryo, expressed on the apical side of neuroepithelial cells and of other epithelia such as lung buds, gut and ureter buds. In the adult, expressed at the apical side of the kidney tubules and of the ependymal layer of the brain. Not expressed in gut, liver, lung, pituitary, adrenal, heart or spleen. Localized to the nascent disk membranes at the base of the rod outer segment in the retina (at protein level)

Product Specifications

Recombinant Mouse Prominin-1 (Prom1), partial is a recombinant protein from Mus musculus (Mouse), expressed in E.coli, covering amino acids 509-794aa, with N-terminal 6XHis-SUMO-tagged tag, molecular weight 48.5kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

May play a role in cell differentiation, proliferation and apoptosis. Binds cholesterol in cholesterol-containing plasma membrane microdomains and may play a role in the organization of the apical plasma membrane in epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis (PubMed:19228982). Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner

Subcellular Location

Apical cell membrane (Multi-pass membrane protein); Cell projection, microvillus membrane (Multi-pass membrane protein); Cell projection, cilium, photoreceptor outer segment; Endoplasmic reticulum; Endoplasmic reticulum-Golgi intermediate compartment

Tissue Specificity

In the submandibular gland, expressed on the apical side of epithelial cells. In the parotid gland, expressed in the intercalated ducts. In the sublingual gland, expressed in intercalated ducts. In the extraorbital lacrimal gland, expressed in the intercalated tubules and larger intralobular ducts. Expressed in the retina. Present in urine within small membrane particles (at protein level). In the embryo, expressed on the apical side of neuroepithelial cells and of other epithelia such as lung buds, gut and ureter buds. In the adult, expressed at the apical side of the kidney tubules and of the ependymal layer of the brain. Not expressed in gut, liver, lung, pituitary, adrenal, heart or spleen. Localized to the nascent disk membranes at the base of the rod outer segment in the retina (at protein level)

Subunit

Interacts with CDHR1 and with actin filaments. Interacts with NAT8 and NAT8B (By similarity)

Gene: Prom1  |  Organism: Mus musculus  |  Synonyms: Antigen AC133 homolog; Prominin-like protein 1
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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