Recombinant Pseudomonas aeruginosa Type III needle protein PscF (pscF), Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 16.6kDa, purity >85% as determined by SDS-PAGE.

Recombinant Pseudomonas aeruginosa Type III needle protein PscF (pscF)

Recombinant Pseudomonas aeruginosa Type III needle protein PscF (pscF) — Component of the type III secretion system (T3SS), also called injectisome, which is used to inject bacterial effector proteins into eukaryotic host cells, facilitating the establishment and dissem… Purity >85%.

SKU: BCRECP-004188 Category:
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Product Specifications

Product SkuBCRECP-004188
Product DescriptionRecombinant Pseudomonas aeruginosa Type III needle protein PscF (pscF) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 2-85aa. [Accession | P95434].
Uniprot No.P95434
Gene NamespscF
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region2-85aa
SpeciesPseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight16.6kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Component of the type III secretion system (T3SS), also called injectisome, which is used to inject bacterial effector proteins into eukaryotic host cells, facilitating the establishment and dissemination of infection. PscF/SctF forms the external needle filament that protrudes from the bacterial surface.

Biological Context

Subcellular Location: Secreted; Cell surface

Product Specifications

Recombinant Pseudomonas aeruginosa Type III needle protein PscF (pscF) is a recombinant protein from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1), expressed in E.coli, covering amino acids 2-85aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 16.6kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Component of the type III secretion system (T3SS), also called injectisome, which is used to inject bacterial effector proteins into eukaryotic host cells, facilitating the establishment and dissemination of infection. PscF/SctF forms the external needle filament that protrudes from the bacterial surface

Subcellular Location

Secreted; Cell surface

Subunit

The core secretion machinery of the T3SS is composed of approximately 20 different proteins, including cytoplasmic components, a base, an export apparatus and a needle (By similarity). This subunit polymerizes and forms the helical needle filament (PubMed:16115870). Forms a stable heterotrimeric complex with PscE and PscG in the cytoplasm, blocking it in a monomeric state and preventing its polymerization (PubMed:16115870, PubMed:17470796)

Gene: sctF  |  Organism: Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)  |  Synonyms: Pseudomonas secretion protein F; Type III needle protein PscF
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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