Recombinant Arabidopsis thaliana Root meristem growth factor 1 (RGF1), Arabidopsis thaliana (Mouse-ear cress), E.coli, N-terminal GST-tagged, 28.2kDa, purity >85% as determined by SDS-PAGE.
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Recombinant Arabidopsis thaliana Root meristem growth factor 1 (RGF1)

Recombinant Arabidopsis thaliana Root meristem growth factor 1 (RGF1) — Acts as a peptide hormone recognized by receptors (e. Purity >85%.

SKU: BCRECP-004268 Categories: ,
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Product Specifications

Product SkuBCRECP-004268
Product DescriptionRecombinant Arabidopsis thaliana Root meristem growth factor 1 (RGF1) Protein is expressed from E.coli with N-terminal GST-tagged. It contains 104-116aa. [Accession | Q3E880].
Uniprot No.Q3E880
Gene NamesRGF1
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region104-116aa
SpeciesArabidopsis thaliana (Mouse-ear cress)
Tag InfoN-terminal GST-tagged
Molecular weight28.2kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasSignal Transduction

Function

Acts as a peptide hormone recognized by receptors (e.g. RGI1 and RGI2) to trigger signaling events including the regulation of RITF1 expression and leading to the production of reactive oxygen species (ROS) in roots to modulate meristem size. Signaling peptide (root growth factor) that maintains the postembryonic root stem cell niche in a PIN2-traffic dependent manner by regulating the expression levels and patterns of the transcription factor PLETHORA (PLT), mainly at the post-transcriptional level.

Biological Context

Subcellular Location: Secreted
Tissue Specificity: Expressed in root tips, only in the quiescent center and the columella stem cells

Product Specifications

Recombinant Arabidopsis thaliana Root meristem growth factor 1 (RGF1) is a recombinant protein from Arabidopsis thaliana (Mouse-ear cress), expressed in E.coli, covering amino acids 104-116aa, with N-terminal GST-tagged tag, molecular weight 28.2kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Acts as a peptide hormone recognized by receptors (e.g. RGI1 and RGI2) to trigger signaling events including the regulation of RITF1 expression and leading to the production of reactive oxygen species (ROS) in roots to modulate meristem size (PubMed:31801996). Signaling peptide (root growth factor) that maintains the postembryonic root stem cell niche in a PIN2-traffic dependent manner by regulating the expression levels and patterns of the transcription factor PLETHORA (PLT), mainly at the post-transcriptional level (PubMed:20798316, PubMed:23370719). Maintains the postembryonic root stem cell niche by regulating the expression levels and patterns of the transcription factor PLETHORA (e.g. PLT1 and PLT2), mainly at the post-transcriptional level (PubMed:20798316). Influences circumferential cell number in the root meristem in response to Pi-deprivation (PubMed:25856240). Regulates the pattern of root growth and lateral root development by modulating the length and the number of cortical cells in the root apical meristem (RAM), and the anticlinal asymmetric cell divisions in lateral root initiation cells (PubMed:22307643, PubMed:23370719)

Subcellular Location

Secreted

Tissue Specificity

Expressed in root tips, only in the quiescent center and the columella stem cells

Subunit

Interacts with RGI1; this interaction triggers RGI1 phosphorylation and ubiquitination (PubMed:27229312). Binds to LRR receptor-like serine/threonine-protein kinases RGI1, RGI2 and RGI3 to trigger their dimerization with SERK proteins and subsequent signaling (PubMed:27001831, PubMed:27229311)

Gene: GLV11  |  Organism: Arabidopsis thaliana  |  Synonyms: CLAVATA3/ESR (CLE)-related protein CLEL8; Root meristem growth factor 1
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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