Recombinant Drosophila melanogaster DNA-directed RNA polymerase II subunit RPB1 (RpII215), partial, Drosophila melanogaster (Fruit fly), Yeast, N-terminal 6xHis-tagged, 33.6kDa, purity >90% as determined by SDS-PAGE.

Recombinant Drosophila melanogaster DNA-directed RNA polymerase II subunit RPB1 (RpII215), partial

Recombinant Drosophila melanogaster DNA-directed RNA polymerase II subunit RPB1 (RpII215), partial — DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Purity >90%.

SKU: BCRECP-004283 Category:
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Product Specifications

Product SkuBCRECP-004283
Product DescriptionRecombinant Drosophila melanogaster DNA-directed RNA polymerase II subunit RPB1 (RpII215) Protein is expressed from Yeast with N-terminal 6xHis-tagged. It contains 1579-1881aa. [Accession | P04052].
Uniprot No.P04052
Gene NamesRpII215
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemYeast
Expression Region1579-1881aa
SpeciesDrosophila melanogaster (Fruit fly)
Tag InfoN-terminal 6xHis-tagged
Molecular weight33.6kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Forms the polymerase active center together with the second largest subunit.

Biological Context

Subcellular Location: Nucleus

Product Specifications

Recombinant Drosophila melanogaster DNA-directed RNA polymerase II subunit RPB1 (RpII215), partial is a recombinant protein from Drosophila melanogaster (Fruit fly), expressed in Yeast, covering amino acids 1579-1881aa, with N-terminal 6xHis-tagged tag, molecular weight 33.6kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. At the start of transcription, a single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol II. A bridging helix emanates from RPB1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol II by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition. During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing (By similarity)

Subcellular Location

Nucleus

Subunit

Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits

Gene: Polr2A  |  Organism: Drosophila melanogaster  |  Synonyms: DNA-directed RNA polymerase III largest subunit; RNA polymerase II subunit A
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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