Recombinant Human coronavirus HKU1 Spike glycoprotein (S), partial, Human coronavirus HKU1 (isolate N2) (HCoV-HKU1), E.coli, C-terminal 6xHis-tagged, 35.7kDa, purity >90% as determined by SDS-PAGE.
, , ,

Recombinant Human coronavirus HKU1 Spike glycoprotein (S), partial

Recombinant Human coronavirus HKU1 Spike glycoprotein (S), partial — recombinant protein from Human coronavirus HKU1 (isolate N2) (HCoV-HKU1), expressed in E.coli. Purity >90%.

SKU: BCRECP-004322 Categories: , , ,
Online Inquiry →

Product Specifications

Product SkuBCRECP-004322
Product DescriptionRecombinant Human coronavirus HKU1 Spike glycoprotein (S) Protein is expressed from E.coli with C-terminal 6xHis-tagged. It contains 310-622aa. [Accession | Q14EB0].
Uniprot No.Q14EB0
Gene NamesS
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region310-622aa
SpeciesHuman coronavirus HKU1 (isolate N2) (HCoV-HKU1)
Tag InfoC-terminal 6xHis-tagged
Molecular weight35.7kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasMicrobiology

Function

Acts as a viral fusion peptide which is unmasked following S2 cleavage occurring upon virus endocytosis.

Biological Context

Subcellular Location: Virion membrane (Single-pass type I membrane protein); Host endoplasmic reticulum-Golgi intermediate compartment membrane (Single-pass type I membrane protein); Host cell membrane (Single-pass type I membrane protein)

Product Specifications

Recombinant Human coronavirus HKU1 Spike glycoprotein (S), partial is a recombinant protein from Human coronavirus HKU1 (isolate N2) (HCoV-HKU1), expressed in E.coli, covering amino acids 310-622aa, with C-terminal 6xHis-tagged tag, molecular weight 35.7kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Acts as a viral fusion peptide which is unmasked following S2 cleavage occurring upon virus endocytosis

Subcellular Location

Virion membrane (Single-pass type I membrane protein); Host endoplasmic reticulum-Golgi intermediate compartment membrane (Single-pass type I membrane protein); Host cell membrane (Single-pass type I membrane protein)

Subunit

Homotrimer; each monomer consists of a S1 and a S2 subunit. The resulting peplomers protrude from the virus surface as spikes

Gene: S  |  Organism: Human coronavirus HKU1 (isolate N2)  |  Synonyms: E2; Peplomer protein

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

Shopping Cart
Scroll to Top