Recombinant Bovine Protein S100-B (S100B), Bos taurus (Bovine), E.coli, N-terminal 6xHis-Avi-tagged, 13.3kDa, purity >90% as determined by SDS-PAGE.

Recombinant Bovine Protein S100-B (S100B)

Recombinant Bovine Protein S100-B (S100B) — Small zinc- and- and calcium-binding protein that is highly expressed in astrocytes and constitutes one of the most abundant soluble proteins in brain. Purity >90%.

SKU: BCRECP-004352 Category:
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Product Specifications

Product SkuBCRECP-004352
Product DescriptionRecombinant Bovine Protein S100-B (S100B) Protein is expressed from E.coli with N-terminal 6xHis-Avi-tagged. It contains 2-92aa. [Accession | P02638].
Uniprot No.P02638
Gene NamesS100B
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region2-92aa
SpeciesBos taurus (Bovine)
Tag InfoN-terminal 6xHis-Avi-tagged
Molecular weight13.3kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasCell Biology

Function

Small zinc- and- and calcium-binding protein that is highly expressed in astrocytes and constitutes one of the most abundant soluble proteins in brain. Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites.

Biological Context

Subcellular Location: Cytoplasm; Nucleus; Secreted

Product Specifications

Recombinant Bovine Protein S100-B (S100B) is a recombinant protein from Bos taurus (Bovine), expressed in E.coli, covering amino acids 2-92aa, with N-terminal 6xHis-Avi-tagged tag, molecular weight 13.3kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Small zinc- and- and calcium-binding protein that is highly expressed in astrocytes and constitutes one of the most abundant soluble proteins in brain (PubMed:3722149, PubMed:6615778). Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer (PubMed:3722149, PubMed:6615778). Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites (PubMed:3722149). Acts as a neurotrophic factor that promotes astrocytosis and axonal proliferation (By similarity). Involved in innervation of thermogenic adipose tissue by acting as an adipocyte-derived neurotrophic factor that promotes sympathetic innervation of adipose tissue (By similarity). Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase (PubMed:14661952). Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling (By similarity). Could assist ATAD3A cytoplasmic processing, preventing aggregation and favoring mitochondrial localization (By similarity). May mediate calcium-dependent regulation on many physiological processes by interacting with other proteins, such as TPR-containing proteins, and modulating their activity (By similarity)

Subcellular Location

Cytoplasm; Nucleus; Secreted

Subunit

Dimer of either two alpha chains, or two beta chains, or one alpha and one beta chain (PubMed:14661952). The S100B dimer binds two molecules of STK38 (PubMed:14661952). Interacts with CACYBP in a calcium-dependent manner (By similarity). Interacts with ATAD3A; this interaction probably occurs in the cytosol prior to ATAD3A mitochondrial targeting (By similarity). Interacts with S100A6 (By similarity). The S100B dimer interacts with two molecules of CAPZA1 (By similarity). Interacts with AGER (By similarity). Interacts with PPP5C (via TPR repeats); the interaction is calcium-dependent and modulates PPP5C activity (By similarity). Interacts with TPPP; this interaction inhibits TPPP dimerization (By similarity). Interacts with isoform CLSTN3beta of CLSTN3; interaction promotes secretion (By similarity)

Gene: S100B  |  Organism: Bos taurus  |  Synonyms: S-100 protein beta chain; S-100 protein subunit beta; S100 calcium-binding protein B
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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