Recombinant Arabidopsis thaliana FACT complex subunit SPT16 (SPT16), partial, Arabidopsis thaliana (Mouse-ear cress), E.coli, N-terminal 6xHis-SUMO-tagged, 36.9kDa, purity >90% as determined by SDS-PAGE.

Recombinant Arabidopsis thaliana FACT complex subunit SPT16 (SPT16), partial

Recombinant Arabidopsis thaliana FACT complex subunit SPT16 (SPT16), partial — Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. Purity >90%.

SKU: BCRECP-004478 Category:
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Product Specifications

Product SkuBCRECP-004478
Product DescriptionRecombinant Arabidopsis thaliana FACT complex subunit SPT16 (SPT16) Protein is expressed from E.coli with N-terminal 6xHis-SUMO-tagged. It contains 890-1074aa. [Accession | O82491].
Uniprot No.O82491
Gene NamesSPT16
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region890-1074aa
SpeciesArabidopsis thaliana (Mouse-ear cress)
Tag InfoN-terminal 6xHis-SUMO-tagged
Molecular weight36.9kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasDevelopmental Biology

Function

Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure.

Biological Context

Subcellular Location: Nucleus; Chromosome
Tissue Specificity: Widely expressed. Present in embryos, shoots and roots, whereas it is not present in terminally differentiated cells such as mature trichoblasts or cells of the root cap (at protein level)

Product Specifications

Recombinant Arabidopsis thaliana FACT complex subunit SPT16 (SPT16), partial is a recombinant protein from Arabidopsis thaliana (Mouse-ear cress), expressed in E.coli, covering amino acids 890-1074aa, with N-terminal 6xHis-SUMO-tagged tag, molecular weight 36.9kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II (Probable)

Subcellular Location

Nucleus; Chromosome

Tissue Specificity

Widely expressed. Present in embryos, shoots and roots, whereas it is not present in terminally differentiated cells such as mature trichoblasts or cells of the root cap (at protein level)

Subunit

Component of the FACT complex, a stable heterodimer of SPT16 and SSRP

Gene: SPT16  |  Organism: Arabidopsis thaliana  |  Synonyms: Facilitates chromatin transcription complex subunit SPT16

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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