Recombinant Sulfolobus solfataricus DNA-binding protein 7d (sso7d), Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2), E.coli, N-terminal 6xHis-SUMO-tagged, 23.1kDa, purity >90% as determined by SDS-PAGE.

Recombinant Sulfolobus solfataricus DNA-binding protein 7d (sso7d)

Recombinant Sulfolobus solfataricus DNA-binding protein 7d (sso7d) — Can constrain negative DNA supercoils. Purity >90%.

SKU: BCRECP-004487 Category:
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Product Specifications

Product SkuBCRECP-004487
Product DescriptionRecombinant Sulfolobus solfataricus DNA-binding protein 7d (sso7d) Protein is expressed from E.coli with N-terminal 6xHis-SUMO-tagged. It contains 2-64aa. [Accession | P39476].
Uniprot No.P39476
Gene Namessso7d
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region2-64aa
SpeciesSulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Tag InfoN-terminal 6xHis-SUMO-tagged
Molecular weight23.1kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Can constrain negative DNA supercoils. May be involved in maintaining the integrity of the genome at high temperature. Stimulates the Holliday junction cleavage activity of Hjc.

Biological Context

Subcellular Location: Cytoplasm

Product Specifications

Recombinant Sulfolobus solfataricus DNA-binding protein 7d (sso7d) is a recombinant protein from Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2), expressed in E.coli, covering amino acids 2-64aa, with N-terminal 6xHis-SUMO-tagged tag, molecular weight 23.1kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Can constrain negative DNA supercoils. May be involved in maintaining the integrity of the genome at high temperature (By similarity). Stimulates the Holliday junction cleavage activity of Hjc (PubMed:11709558)

Subcellular Location

Cytoplasm

Subunit

Monomer

Gene: sso7d  |  Organism: Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)  |  Synonyms: 7 kDa DNA-binding protein d; Sso7d
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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