Recombinant Enterobacteria phage K3 Holin (T), partial, Enterobacteria phage K3 (Bacteriophage K3), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 27.2kDa, purity >90% as determined by SDS-PAGE.

Recombinant Enterobacteria phage K3 Holin (T), partial

Recombinant Enterobacteria phage K3 Holin (T), partial — Accumulates harmlessly in the cytoplasmic membrane until it reaches a critical concentration that triggers the formation of micron-scale pores (holes) causing host cell membrane disruption and endo… Purity >90%.

SKU: BCRECP-004523 Category:
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Product Specifications

Product SkuBCRECP-004523
Product DescriptionRecombinant Enterobacteria phage K3 Holin (T) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 50-218aa. [Accession | P10393].
Uniprot No.P10393
Gene NamesT
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region50-218aa
SpeciesEnterobacteria phage K3 (Bacteriophage K3)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight27.2kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Accumulates harmlessly in the cytoplasmic membrane until it reaches a critical concentration that triggers the formation of micron-scale pores (holes) causing host cell membrane disruption and endolysin escape into the periplasmic space. Determines the precise timing of host cell lysis. Regulated by specific antiholins that somehow sense superinfections and then delay lysis.

Biological Context

Subcellular Location: Host cell inner membrane (Single-pass type II membrane protein)

Product Specifications

Recombinant Enterobacteria phage K3 Holin (T), partial is a recombinant protein from Enterobacteria phage K3 (Bacteriophage K3), expressed in E.coli, covering amino acids 50-218aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 27.2kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Accumulates harmlessly in the cytoplasmic membrane until it reaches a critical concentration that triggers the formation of micron-scale pores (holes) causing host cell membrane disruption and endolysin escape into the periplasmic space. Determines the precise timing of host cell lysis. Regulated by specific antiholins that somehow sense superinfections and then delay lysis. Participates with the endolysin and spanin proteins in the sequential events which lead to the programmed host cell lysis releasing the mature viral particles from the host cell

Subcellular Location

Host cell inner membrane (Single-pass type II membrane protein)

Subunit

Homomultimer. Heterotetramer composed of 2 holin and 2 antiholin. The holin-antiholin complex binds dsDNA. Interacts (via C-terminus) with antiholin (via C-terminus); this interaction blocks the holin homomultimerization and delays host cell lysis. Interacts (via N-terminus) with the lysis inhibition accessory protein rIII; this interaction stabilizes the holin-antiholin complex thereby resulting in a robust block of the hole formation

Gene: T  |  Organism: Enterobacteria phage K3  |  Synonyms: Lysis protein

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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