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Recombinant Human T-cell leukemia virus 1 Protein Tax-1 (Tax)

Recombinant Human T-cell leukemia virus 1 Protein Tax-1 (Tax) — Transcriptional activator that governs the viral transcription from the 5’LTR via the recruitment of dimers of host phosphorylated CREB1. Purity >85%.

SKU: BCRECP-004527 Categories: ,
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Product Specifications

Product SkuBCRECP-004527
Product DescriptionRecombinant Human T-cell leukemia virus 1 Protein Tax-1 (Tax) Protein is expressed from E.coli with N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged. It contains 1-353aa. [Accession | P03409].
Uniprot No.P03409
Gene Namestax
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-353aa
SpeciesHuman T-cell leukemia virus 1 (strain Japan ATK-1 subtype A) (HTLV-1)
Tag InfoN-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged
Molecular weight55.5kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasCancer

Function

Transcriptional activator that governs the viral transcription from the 5'LTR via the recruitment of dimers of host phosphorylated CREB1. Together they bind cAMP response elements within the viral promoter and mediate high-level viral transcription. Increases host CREB1 O-GlcNAcylation to further increase 5'LTR transactivation.

Biological Context

Subcellular Location: Host nucleus; Host cytoplasm

Product Specifications

Recombinant Human T-cell leukemia virus 1 Protein Tax-1 (Tax) is a recombinant protein from Human T-cell leukemia virus 1 (strain Japan ATK-1 subtype A) (HTLV-1), expressed in E.coli, covering amino acids 1-353aa, with N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged tag, molecular weight 55.5kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Transcriptional activator that governs the viral transcription from the 5'LTR via the recruitment of dimers of host phosphorylated CREB1. Together they bind cAMP response elements within the viral promoter and mediate high-level viral transcription (PubMed:22789739, PubMed:8970957). Increases host CREB1 O-GlcNAcylation to further increase 5'LTR transactivation (PubMed:28742148). Modulates also the expression of cellular genes leading to the deregulation of T-cell proliferation, perturbing the integrity of cell cycle checkpoints, the DNA damage response and apopototic pathways. Acts as an ubiquitin E3 ligase and stimulates host IKK complex by catalyzing the assembly of free mixed-linkage polyubiquitin chains, resulting in constitutive activation of the transcription factor NF-kappa-B (PubMed:27082114, PubMed:28103322). Inhibits the host nonsense-mediated mRNA decay (NMD), a cellular process that can actively degrade mRNAs by interacting with host UPF1 (PubMed:27082114)

Subcellular Location

Host nucleus; Host cytoplasm

Subunit

Homodimer. Interacts with host CREB1 (PubMed:8970957). Interacts with host DLG1, IKBKG, KDM4A, MAGII3 and TAX1BP1. Recruits the coactivators CREBBP, EP300 and PCAF (PubMed:10766811). Interaction with human CARM1 enhances Tax transactivation and promotes methylation of histone H3 (PubMed:17005681). Interacts with host SUV39H1 protein, leading to abrogate Tax transactivation of HTLV-1 LTR (PubMed:16409643). Interaction with human CREB coactivators, CRTC1/TORC1, CRTC2/TORC2 and CRTC3/TORC3 enhances Tax transactivation (PubMed:15466468, PubMed:16809310). Interacts with host UPF1; this interaction inhibits the host nonsense-mediated mRNA decay (NMD). Interacts (via N-terminus) with host PLSCR1; this interaction negatively regulates Tax transactivation activity by altering its subcellular distribution and homodimerization (PubMed:22789739)

Gene: Tax  |  Organism: Human T-cell leukemia virus 1 (strain Japan ATK-1 subtype A)  |  Synonyms: Protein X-LOR; Trans-activating transcriptional regulatory protein of HTLV-1
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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