Recombinant Clostridium tetani Tetanus toxin (tetX), partial, Clostridium tetani (strain Massachusetts / E88), E.coli, N-terminal 10xHis-tagged, 105.1kDa, purity >90% as determined by SDS-PAGE.

Recombinant Clostridium tetani Tetanus toxin (tetX), partial

Recombinant Clostridium tetani Tetanus toxin (tetX), partial — Tetanus toxin acts by inhibiting neurotransmitter release. Purity >90%.

SKU: BCRECP-004542 Category:
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Product Specifications

Product SkuBCRECP-004542
Product DescriptionRecombinant Clostridium tetani Tetanus toxin (tetX) Protein is expressed from E.coli with N-terminal 10xHis-tagged. It contains 1-864aa. [Accession | P04958].
Uniprot No.P04958
Gene NamestetX
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-864aa
SpeciesClostridium tetani (strain Massachusetts / E88)
Tag InfoN-terminal 10xHis-tagged
Molecular weight105.1kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Tetanus toxin acts by inhibiting neurotransmitter release. It binds to peripheral neuronal synapses, is internalized and moves by retrograde transport up the axon into the spinal cord where it can move between postsynaptic and presynaptic neurons. It inhibits neurotransmitter release by acting as a zinc endopeptidase that catalyzes the hydrolysis of the '76-Gln-|-Phe-77' bond of synaptobrevin-2.

Product Specifications

Recombinant Clostridium tetani Tetanus toxin (tetX), partial is a recombinant protein from Clostridium tetani (strain Massachusetts / E88), expressed in E.coli, covering amino acids 1-864aa, with N-terminal 10xHis-tagged tag, molecular weight 105.1kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Tetanus toxin acts by inhibiting neurotransmitter release. It binds to peripheral neuronal synapses, is internalized and moves by retrograde transport up the axon into the spinal cord where it can move between postsynaptic and presynaptic neurons. It inhibits neurotransmitter release by acting as a zinc endopeptidase that catalyzes the hydrolysis of the '76-Gln-|-Phe-77' bond of synaptobrevin-2

Subunit

The precursor polypeptide is subsequently cleaved to yield subchains L and H. These remain linked by a disulfide bridge and are non-toxic after separation

Gene: tetX  |  Organism: Clostridium tetani (strain Massachusetts / E88)  |  Synonyms: Tentoxylysin
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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