Function
Part of the ATP-binding cassette (ABC) transport system TroABC involved in zinc import. Binds zinc with high affinity and specificity and delivers it to the membrane permease for translocation into the cytoplasm.
Biological Context
Subcellular Location: Periplasm
Product Specifications
Recombinant Treponema pallidum Periplasmic zinc-binding protein troA (troA) is a recombinant protein from Treponema pallidum (strain Nichols), expressed in E.coli, covering amino acids 23-308aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 38.2kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.
SDS-PAGE: Single band at expected molecular weight confirming purity.
ELISA: Suitable as coating antigen or detection standard.
Western Blot: Compatible with standard Western Blot protocols.
Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.
Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.
Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.
Shelf Life: 12 months from date of receipt when stored as recommended.
Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.