Recombinant Escherichia phage T7 T7 RNA polymerase (1), partial, Escherichia phage T7 (Bacteriophage T7), E.coli, N-terminal 6xHis-KSI-tagged, 26.5kDa, purity >85% as determined by SDS-PAGE.

Recombinant Escherichia phage T7 T7 RNA polymerase (1), partial

Recombinant Escherichia phage T7 T7 RNA polymerase (1), partial — Highly processive DNA-dependent RNA polymerase that catalyzes the transcription of class II and class III viral genes. Purity >85%.

SKU: BCRECP-001955 Category:
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Product Specifications

Product SkuBCRECP-001955
Product DescriptionRecombinant Escherichia phage T7 T7 RNA polymerase (1) Protein is expressed from E.coli with N-terminal 6xHis-KSI-tagged. It contains 710-805aa. [Accession | P00573].
Uniprot No.P00573
Gene Names1
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region710-805aa
SpeciesEscherichia phage T7 (Bacteriophage T7)
Tag InfoN-terminal 6xHis-KSI-tagged
Molecular weight26.5kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasSignal Transduction

Function

Highly processive DNA-dependent RNA polymerase that catalyzes the transcription of class II and class III viral genes. Recognizes a specific promoter sequence and enters first into an 'abortive phase' where very short transcripts are synthesized and released before proceeding to the processive transcription of long RNA chains. Unwinds the double-stranded DNA to expose the coding strand for templating.

Product Specifications

Recombinant Escherichia phage T7 T7 RNA polymerase (1), partial is a recombinant protein from Escherichia phage T7 (Bacteriophage T7), expressed in E.coli, covering amino acids 710-805aa, with N-terminal 6xHis-KSI-tagged tag, molecular weight 26.5kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Highly processive DNA-dependent RNA polymerase that catalyzes the transcription of class II and class III viral genes. Recognizes a specific promoter sequence and enters first into an 'abortive phase' where very short transcripts are synthesized and released before proceeding to the processive transcription of long RNA chains. Unwinds the double-stranded DNA to expose the coding strand for templating. Participates in the initiation of viral DNA replication presumably by making primers accessible to the DNA polymerase, thus facilitating the DNA opening. Also plays a role in viral DNA packaging, probably by pausing the transcription at the right end of concatemer junction to allow packaging complex recruitment and beginning of the packaging process

Subunit

Monomer. Interacts with T7 lysozyme; this interaction inhibits transcriptional function of T7 RNA polymerase

Organism: Escherichia phage T7  |  Synonyms: DNA-directed RNA polymerase
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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