Recombinant Mouse RAC-beta serine/threonine-protein kinase (Akt2), Mus musculus (Mouse), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 63.2kDa, purity >85% as determined by SDS-PAGE.
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Recombinant Mouse RAC-beta serine/threonine-protein kinase (Akt2)

Recombinant Mouse RAC-beta serine/threonine-protein kinase (Akt2) — Several AKT2-specific substrates have been identified, including ANKRD2, C2CD5, CLK2 and PITX2. Purity >85%.

SKU: BCRECP-002049 Categories: , ,
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Product Specifications

Product SkuBCRECP-002049
Product DescriptionRecombinant Mouse RAC-beta serine/threonine-protein kinase (Akt2) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 1-481aa. [Accession | Q60823].
Uniprot No.Q60823
Gene NamesAkt2
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-481aa
SpeciesMus musculus (Mouse)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight63.2kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasCancer

Function

Several AKT2-specific substrates have been identified, including ANKRD2, C2CD5, CLK2 and PITX2. May play a role in myoblast differentiation. In this context, may act through PITX2 phosphorylation.

Biological Context

Subcellular Location: Cytoplasm; Nucleus; Cell membrane (Peripheral membrane protein); Early endosome

Product Specifications

Recombinant Mouse RAC-beta serine/threonine-protein kinase (Akt2) is a recombinant protein from Mus musculus (Mouse), expressed in E.coli, covering amino acids 1-481aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 63.2kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Several AKT2-specific substrates have been identified, including ANKRD2, C2CD5, CLK2 and PITX2. May play a role in myoblast differentiation (PubMed:17565718). In this context, may act through PITX2 phosphorylation. Unphosphorylated PITX2 associates with an ELAVL1/HuR-containing complex, which stabilizes cyclin mRNA and ensuring cell proliferation. Phosphorylation by AKT2 impairs this association, leading to CCND1 mRNA destabilization and progression towards differentiation (PubMed:20019746). Also involved in the negative regulation of myogenesis in response to stress conditions. In this context, acts by phosphorylating ANKRD2 (PubMed:21737686). May also be a key regulator of glucose uptake. Regulates insulin-stimulated glucose transport by the increase of glucose transporter GLUT4 translocation from intracellular stores to the plasma membrane. In this context, acts by phosphorylating C2CD5/CDP138 on 'Ser-197' in insulin-stimulated adipocytes (PubMed:21907143). Through the phosphorylation of CLK2 on 'Thr-343', involved in insulin-regulated suppression of hepatic gluconeogenesis (PubMed:20074525)

Subcellular Location

Cytoplasm; Nucleus; Cell membrane (Peripheral membrane protein); Early endosome

Subunit

Interacts with BTBD10 (PubMed:18160256). Interacts with KCTD20 (PubMed:24156551). Interacts (via PH domain) with MTCP1, TCL1A and TCL1B; this interaction may facilitate AKT2 oligomerization and phosphorylation, hence increasing kinase activity (By similarity). Interacts with PHB2; this interaction may be important for myogenic differentiation (PubMed:17565718). Interacts (when phosphorylated) with CLIP3/ClipR-59; this interaction promotes cell membrane localization (PubMed:19139280). Interacts with WDFY2 (via WD repeats 1-3) (PubMed:16792529, PubMed:20189988)

Gene: Akt2  |  Organism: Mus musculus  |  Synonyms: Protein kinase Akt-2; Protein kinase B beta; RAC-PK-beta
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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