Recombinant Rat Apelin (Apln), partial, Rattus norvegicus (Rat), Yeast, N-terminal 6xHis-tagged, 2.4kDa, purity >95% as determined by SDS-PAGE.

Recombinant Rat Apelin (Apln), partial

Recombinant Rat Apelin (Apln), partial — Peptide hormone that functions as endogenous ligand for the G protein-coupled apelin receptor (APLNR/APJ), that plays a role in cadiovascular homeostasis. Purity >95%.

SKU: BCRECP-002107 Category:
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Product Specifications

Product SkuBCRECP-002107
Product DescriptionRecombinant Rat Apelin (Apln) Protein is expressed from Yeast with N-terminal 6xHis-tagged. It contains 65-77aa. [Accession | Q9R0R3].
Uniprot No.Q9R0R3
Gene NamesApln
PurityGreater than 95% as determined by SDS-PAGE.
Expression SystemYeast
Expression Region65-77aa
SpeciesRattus norvegicus (Rat)
Tag InfoN-terminal 6xHis-tagged
Molecular weight2.4kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasCardiovascular

Function

Peptide hormone that functions as endogenous ligand for the G protein-coupled apelin receptor (APLNR/APJ), that plays a role in cadiovascular homeostasis. Functions as a balanced agonist activating both G(i) protein pathway and beta-arrestin pathway of APLNR.

Product Specifications

Recombinant Rat Apelin (Apln), partial — Peptide hormone that functions as endogenous ligand for the G protein-coupled apelin receptor (APLNR/APJ), that plays a role in cadiovascular homeostasis. Purity >95%. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Peptide hormone that functions as endogenous ligand for the G protein-coupled apelin receptor (APLNR/APJ), that plays a role in cadiovascular homeostasis (PubMed:11336787, PubMed:11359874, PubMed:26611206). Functions as a balanced agonist activating both G(i) protein pathway and beta-arrestin pathway of APLNR (By similarity). Downstream G proteins activation, apelin can inhibit cAMP production and activate key intracellular effectors such as ERKs (PubMed:26611206). On the other hand, APLNR activation induces beta-arrestin recruitment to the membrane leading to desensitization and internalization of the receptor (PubMed:11359874). Apelin blunts cardiac hypertrophic induction from APLNR on response to pathological stimuli, but also induces myocardial hypertrophy under normal conditions (By similarity). Apelin-36 dissociates more hardly than (pyroglu)apelin-13 from APLNR (PubMed:11336787). Involved in the regulation of cardiac precursor cell movements during gastrulation and heart morphogenesis (By similarity). Has an inhibitory effect on cytokine production in response to T-cell receptor/CD3 cross-linking; the oral intake of apelin in the colostrum and the milk might therefore modulate immune responses in neonates. Plays a role in early coronary blood vessels formation (By similarity). Mediates myocardial contractility in an ERK1/2-dependent manner (PubMed:11359874, PubMed:26611206). May also have a role in the central control of body fluid homeostasis by influencing vasopressin release and drinking behavior (PubMed:10617103)

Subcellular Location

Secreted; Secreted, extracellular space

Tissue Specificity

Expressed in the lung, testis, ovary, uterus and mammary gland (PubMed:11336787). Expressed in neurons in the thalamic paraventricular and hypothalamic supraoptic nuclei (PubMed:11359874). The lung, testis and uterus mainly contain a large form that looks like apelin-36, whereas the mammary gland seems to contain 2 forms of apelin, a large form close to apelin-36 and a small form close to apelin-13 (at protein level) (PubMed:11336787). Widely expressed in the adult, with highest levels in the mammary gland of lactating animals, very high levels in the lung, intermediate levels in the spinal cord, ovary, adipose tissue, brain (neuronal cell bodies and fibers in the supraoptic and the paraventricular nuclei), heart and testis, and lowest levels in the pituitary gland, kidney, stomach, uterus and pancreas (PubMed:10525157, PubMed:10617103, PubMed:11336787)

Gene: Apln  |  Organism: Rattus norvegicus  |  Synonyms: APJ endogenous ligand

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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