Recombinant Variola virus Pro-variola growth factor (B3R), partial, Variola virus (isolate Human/India/Ind3/1967) (VARV) (Smallpox virus), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 16.6kDa, purity >85% as determined by SDS-PAGE.
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Recombinant Variola virus Pro-variola growth factor (B3R), partial

Recombinant Variola virus Pro-variola growth factor (B3R), partial — Stimulates cellular proliferation (hyperplasia)and mobility around infected cells to promote rapid and efficient spread of infection. Purity >85%.

SKU: BCRECP-002170 Categories: , ,
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Product Specifications

Product SkuBCRECP-002170
Product DescriptionRecombinant Variola virus Pro-variola growth factor (B3R) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 19-100aa. [Accession | P0DOP9].
Uniprot No.P0DOP9
Gene NamesB3R
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region19-100aa
SpeciesVariola virus (isolate Human/India/Ind3/1967) (VARV) (Smallpox virus)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight16.6kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Stimulates cellular proliferation (hyperplasia)and mobility around infected cells to promote rapid and efficient spread of infection. This effect is beneficial for virus replication in vivo, because poxviruses replicate possibly better in proliferating cells than in quiescent cells. Acts by binding host EGFR, inducing its dimerization, autophosphorylation and leading to activation of several cellular pathways regulating cell proliferation or cell survival.

Biological Context

Subcellular Location: Secreted

Product Specifications

Recombinant Variola virus Pro-variola growth factor (B3R), partial is a recombinant protein from Variola virus (isolate Human/India/Ind3/1967) (VARV) (Smallpox virus), expressed in E.coli, covering amino acids 19-100aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 16.6kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Stimulates cellular proliferation (hyperplasia)and mobility around infected cells to promote rapid and efficient spread of infection. This effect is beneficial for virus replication in vivo, because poxviruses replicate possibly better in proliferating cells than in quiescent cells. Acts by binding host EGFR, inducing its dimerization, autophosphorylation and leading to activation of several cellular pathways regulating cell proliferation or cell survival. The activation by host EGFR of mitogen activated protein kinases (MAPK) and extracellular-signal regulated kinases (ERK) are essential for the positive effect of vaccinia growth factor on poxvirus virulence in vivo

Subcellular Location

Secreted

Subunit

Variola growth factor interacts with host EGFR and promotes EGFR dimerization

Gene: OPG019  |  Organism: Variola virus (isolate Human/India/Ind3/1967)

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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