Recombinant Eschscholzia californica Reticuline oxidase (BBE1), Eschscholzia californica (California poppy), E.coli, N-terminal 6xHis-tagged, 61.4kDa, purity >90% as determined by SDS-PAGE.
,

Recombinant Eschscholzia californica Reticuline oxidase (BBE1)

Recombinant Eschscholzia californica Reticuline oxidase (BBE1) — Essential to the formation of benzophenanthridine alkaloids in the response of plants to pathogenic attack. Purity >90%.

SKU: BCRECP-002183 Categories: ,
Online Inquiry →

Product Specifications

Product SkuBCRECP-002183
Product DescriptionRecombinant Eschscholzia californica Reticuline oxidase (BBE1) Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 24-538aa. [Accession | P30986].
Uniprot No.P30986
Gene NamesBBE1
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region24-538aa
SpeciesEschscholzia californica (California poppy)
Tag InfoN-terminal 6xHis-tagged
Molecular weight61.4kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Essential to the formation of benzophenanthridine alkaloids in the response of plants to pathogenic attack. Catalyzes the stereospecific conversion of the N-methyl moiety of (S)-reticuline into the berberine bridge carbon of (S)-scoulerine.

Biological Context

Subcellular Location: Cytoplasmic vesicle
Pathway: Alkaloid biosynthesis; (S)-scoulerine biosynthesis; (S)-scoulerine from (S)-reticuline: step 1/1

Product Specifications

Recombinant Eschscholzia californica Reticuline oxidase (BBE1) is a recombinant protein from Eschscholzia californica (California poppy), expressed in E.coli, covering amino acids 24-538aa, with N-terminal 6xHis-tagged tag, molecular weight 61.4kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Essential to the formation of benzophenanthridine alkaloids in the response of plants to pathogenic attack. Catalyzes the stereospecific conversion of the N-methyl moiety of (S)-reticuline into the berberine bridge carbon of (S)-scoulerine

Subcellular Location

Cytoplasmic vesicle

Pathway

Alkaloid biosynthesis; (S)-scoulerine biosynthesis; (S)-scoulerine from (S)-reticuline: step 1/1

Gene: BBE1  |  Organism: Eschscholzia californica  |  Synonyms: Berberine bridge-forming enzyme; Tetrahydroprotoberberine synthase
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

Shopping Cart
Scroll to Top