Recombinant Mouse Calreticulin (Calr)

Function

Calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER (PubMed:20880849, PubMed:21652723). Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export (By similarity). Involved in maternal gene expression regulation. May participate in oocyte maturation via the regulation of calcium homeostasis (By similarity). Present in the cortical granules of non-activated oocytes, is exocytosed during the cortical reaction in response to oocyte activation and might participate in the block to polyspermy (By similarity)

Subcellular Location

Endoplasmic reticulum lumen; Cytoplasm, cytosol; Cytolytic granule; Secreted, extracellular space, extracellular matrix; Cell surface; Sarcoplasmic reticulum lumen; Cytoplasmic vesicle, secretory vesicle, Cortical granule

Subunit

Monomer. Interacts with GABARAP, NR3C1, PDIA3/ERp57 and TRIM21. Interacts (via P-domain) with PDIA5 (By similarity). Interacts with PPIB (PubMed:20801878). Interacts with SPACA9 (PubMed:24256100). Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5 (PubMed:16931514). Interacts with CLCC1 (By similarity)

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• Mazzarella R.A. et al. Determination of the sequence of an expressible cDNA clone encoding ERp60/calregulin by the use of a novel nested set method. — Gene (1992)
• Carninci P. et al. The transcriptional landscape of the mammalian genome. — Science (2005)
• The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). — Genome Res. (2004)
• Merrick B.A. et al. Separation and sequencing of familiar and novel murine proteins using preparative two-dimensional gel electrophoresis. — Electrophoresis (1994)
• Lubec G. et al. PubMed: CI-5R8R933V1S8KD (JAN-)
• Dupuis M. et al. The calcium-binding protein calreticulin is a major constituent of lytic granules in cytolytic T lymphocytes. — J. Exp. Med. (1993)
• Timchenko L.T. et al. Age-specific CUGBP1-eIF2 complex increases translation of CCAAT/enhancer-binding protein beta in old liver. — J. Biol. Chem. (2006)