Recombinant Pig Calreticulin (CALR), Sus scrofa (Pig), Yeast, N-terminal 6xHis-tagged, 48.6kDa, purity >90% as determined by SDS-PAGE.

Recombinant Pig Calreticulin (CALR)

Recombinant Pig Calreticulin (CALR) — Calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. Purity >90%.

SKU: BCRECP-002289 Category:
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Product Specifications

Product SkuBCRECP-002289
Product DescriptionRecombinant Pig Calreticulin (CALR) Protein is expressed from Yeast with N-terminal 6xHis-tagged. It contains 18-417aa. [Accession | P28491].
Uniprot No.P28491
Gene NamesCALR
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemYeast
Expression Region18-417aa
SpeciesSus scrofa (Pig)
Tag InfoN-terminal 6xHis-tagged
Molecular weight48.6kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasTags & Cell Markers

Function

Calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export.

Biological Context

Subcellular Location: Endoplasmic reticulum lumen; Cytoplasm, cytosol; Secreted, extracellular space, extracellular matrix; Cell surface; Sarcoplasmic reticulum lumen; Cytoplasmic vesicle, secretory vesicle, Cortical granule; Cytolytic granule
Tissue Specificity: In blastocyst expressed in all blastomeres (at protein level). In embryos, expressed in spleen, kidney, liver, fat, muscle, ovary, granulosa cells and cumulus cells

Product Specifications

Recombinant Pig Calreticulin (CALR) is a recombinant protein from Sus scrofa (Pig), expressed in Yeast, covering amino acids 18-417aa, with N-terminal 6xHis-tagged tag, molecular weight 48.6kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export (By similarity). Involved in maternal gene expression regulation. May participate in oocyte maturation via the regulation of calcium homeostasis (PubMed:20222029). Present in the cortical granules of non-activated oocytes, is exocytosed during the cortical reaction in response to oocyte activation and might participate in the block to polyspermy (By similarity)

Subcellular Location

Endoplasmic reticulum lumen; Cytoplasm, cytosol; Secreted, extracellular space, extracellular matrix; Cell surface; Sarcoplasmic reticulum lumen; Cytoplasmic vesicle, secretory vesicle, Cortical granule; Cytolytic granule

Tissue Specificity

In blastocyst expressed in all blastomeres (at protein level). In embryos, expressed in spleen, kidney, liver, fat, muscle, ovary, granulosa cells and cumulus cells

Subunit

Monomer. Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5. Interacts with PDIA3/ERp57 and SPACA9 (By similarity). Interacts with TRIM21. Interacts with NR3C1. Interacts with PPIB. Interacts (via P-domain) with PDIA5. Interacts with CLCC1 (By similarity)

Gene: CALR  |  Organism: Sus scrofa  |  Synonyms: CRP55; Calregulin; Endoplasmic reticulum resident protein 60; HACBP

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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