Recombinant Acidaminococcus sp. CRISPR-associated endonuclease Cas12a (cas12a), Acidaminococcus sp. (strain BV3L6), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 158.6kDa, purity >90% as determined by SDS-PAGE.

Recombinant Acidaminococcus sp. CRISPR-associated endonuclease Cas12a (cas12a)

Recombinant Acidaminococcus sp. CRISPR-associated endonuclease Cas12a (cas12a) — CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugat… Purity >90%.

SKU: BCRECP-002292 Category:
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Product Specifications

Product SkuBCRECP-002292
Product DescriptionRecombinant Acidaminococcus sp. CRISPR-associated endonuclease Cas12a (cas12a) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 1-1307aa. [Accession | U2UMQ6].
Uniprot No.U2UMQ6
Gene Namescas12a
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-1307aa
SpeciesAcidaminococcus sp. (strain BV3L6)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight158.6kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA).

Product Specifications

Recombinant Acidaminococcus sp. CRISPR-associated endonuclease Cas12a (cas12a) is a recombinant protein from Acidaminococcus sp. (strain BV3L6), expressed in E.coli, covering amino acids 1-1307aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 158.6kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Recognizes a short motif in the CRISPR repeat sequences (the 5' PAM or protospacer adjacent motif, TTTN in this organism) to help distinguish self versus nonself, as targets within the bacterial CRISPR locus do not have PAMs (PubMed:26422227). Has dsDNA endonuclease activity, results in staggered 4-base 5' overhangs 19 and 22 bases downstream of the PAM on the non-targeted and targeted strand respectively (PubMed:26422227). Non-target strand cleavage by the RuvC domain is probably a prerequisite of target strand cleavage by the Nuc domain (PubMed:27114038). Protects E.coli against plasmids and bacteriophage M13mp18, phage T4 with hydroxymethyl or unmodified (but not glycosylated) cytosines and to a lesser extent against lambda and VpaE1 phage (PubMed:38261981). In this CRISPR system correct processing of pre-crRNA requires only this protein and the CRISPR locus (By similarity)

Subunit

Monomer (PubMed:27114038)

Gene: cas12a  |  Organism: Acidaminococcus sp. (strain BV3L6)  |  Synonyms: AsCpf1; CRISPR-associated endonuclease Cpf1
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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