Recombinant Mouse CD40 ligand (Cd40lg), partial (Active), Mus musculus (Mouse), Mammalian cell, N-terminal mFc1-tagged, 42.1kDa, purity >95% as determined by SDS-PAGE.
,

Recombinant Mouse CD40 ligand (Cd40lg), partial (Active)

Recombinant Mouse CD40 ligand (Cd40lg), partial (Active) — recombinant protein from Mus musculus (Mouse), expressed in Mammalian cell. Purity >95%.

SKU: BCRECP-002329 Categories: ,
Online Inquiry →

Product Specifications

Product SkuBCRECP-002329
Product DescriptionRecombinant Mouse CD40 ligand (Cd40lg) Protein is expressed from Mammalian cell with N-terminal mFc1-tagged. It contains 112-260aa. [Accession | P27548].
Uniprot No.P27548
Gene NamesCd40lg
PurityGreater than 95% as determined by SDS-PAGE.
Expression SystemMammalian cell
Expression Region112-260aa
SpeciesMus musculus (Mouse)
Tag InfoN-terminal mFc1-tagged
Molecular weight42.1kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasCancer

Function

Acts as a ligand for integrins, specifically ITGA5:ITGB1 and ITGAV:ITGB3; both integrins and the CD40 receptor are required for activation of CD40-CD40LG signaling, which have cell-type dependent effects, such as B-cell activation, NF-kappa-B signaling and anti-apoptotic signaling.

Biological Context

Subcellular Location: Secreted
Tissue Specificity: Specifically expressed on activated CD4+ T-lymphocytes

Product Specifications

Recombinant Mouse CD40 ligand (Cd40lg), partial (Active) is a recombinant protein from Mus musculus (Mouse), expressed in Mammalian cell, covering amino acids 112-260aa, with N-terminal mFc1-tagged tag, molecular weight 42.1kDa, purity Greater than 95% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Acts as a ligand for integrins, specifically ITGA5:ITGB1 and ITGAV:ITGB3; both integrins and the CD40 receptor are required for activation of CD40-CD40LG signaling, which have cell-type dependent effects, such as B-cell activation, NF-kappa-B signaling and anti-apoptotic signaling

Subcellular Location

Secreted

Tissue Specificity

Specifically expressed on activated CD4+ T-lymphocytes

Subunit

Homotrimer (PubMed:8095800). Interacts with CD28 (By similarity). CD40 ligand, soluble form: Exists as either a monomer or a homotrimer (By similarity). Forms a ternary complex between CD40 and integrins for CD40-CD40LG signaling (By similarity)

Gene: Cd40lg  |  Organism: Mus musculus  |  Synonyms: T-cell antigen Gp39; TNF-related activation protein; Tumor necrosis factor ligand superfamily member 5

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

Shopping Cart
Scroll to Top