Recombinant Rat Complement factor D (Cfd), Rattus norvegicus (Rat), Mammalian cell, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 29.8kDa, purity >90% as determined by SDS-PAGE.

Recombinant Rat Complement factor D (Cfd)

Recombinant Rat Complement factor D (Cfd) — Serine protease that initiates the alternative pathway of the complement system, a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adapt… Purity >90%.

SKU: BCRECP-002356 Category:
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Product Specifications

Product SkuBCRECP-002356
Product DescriptionRecombinant Rat Complement factor D (Cfd) Protein is expressed from Mammalian cell with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 26-263aa. [Accession | P32038].
Uniprot No.P32038
Gene NamesCfd
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemMammalian cell
Expression Region26-263aa
SpeciesRattus norvegicus (Rat)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight29.8kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasImmunology

Function

Serine protease that initiates the alternative pathway of the complement system, a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system. In contrast to other complement pathways (classical, lectin and GZMK) that are directly activated by pathogens or antigen-antibody complexes, the alternative complement pathway is initiated by the spontaneous hydrolysis of complement C3. The alternative complement pathway acts as an amplification loop that enhances complement activation by mediating the formation of C3 and C5 convertases.

Biological Context

Subcellular Location: Secreted

Product Specifications

Recombinant Rat Complement factor D (Cfd) is a recombinant protein from Rattus norvegicus (Rat), expressed in Mammalian cell, covering amino acids 26-263aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 29.8kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Serine protease that initiates the alternative pathway of the complement system, a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system. In contrast to other complement pathways (classical, lectin and GZMK) that are directly activated by pathogens or antigen-antibody complexes, the alternative complement pathway is initiated by the spontaneous hydrolysis of complement C3. The alternative complement pathway acts as an amplification loop that enhances complement activation by mediating the formation of C3 and C5 convertases. Activated CFD cleaves factor B (CFB) when the latter is complexed with complement C3b, activating the C3 convertase of the alternative pathway

Subcellular Location

Secreted

Gene: Cfd  |  Organism: Rattus norvegicus  |  Synonyms: Adipsin; C3 convertase activator; Endogenous vascular elastase; Properdin factor D

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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