Recombinant Bacillus thuringiensis subsp. kurstaki Pesticidal crystal protein cry1Ac (cry1Ac), partial, Bacillus thuringiensis subsp. kurstaki, Yeast, N-terminal 6xHis-tagged, 25.8kDa, purity >90% as determined by SDS-PAGE.

Recombinant Bacillus thuringiensis subsp. kurstaki Pesticidal crystal protein cry1Ac (cry1Ac), partial

Recombinant Bacillus thuringiensis subsp. kurstaki Pesticidal crystal protein cry1Ac (cry1Ac), partial — recombinant protein from Bacillus thuringiensis subsp. kurstaki, expressed in Yeast. Purity >90%.

SKU: BCRECP-002439 Category:
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Product Specifications

Product SkuBCRECP-002439
Product DescriptionRecombinant Bacillus thuringiensis subsp. kurstaki Pesticidal crystal protein cry1Ac (cry1Ac) Protein is expressed from Yeast with N-terminal 6xHis-tagged. It contains 972-1178aa. [Accession | P05068].
Uniprot No.P05068
Gene Namescry1Ac
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemYeast
Expression Region972-1178aa
SpeciesBacillus thuringiensis subsp. kurstaki
Tag InfoN-terminal 6xHis-tagged
Molecular weight25.8kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Promotes colloidosmotic lysis by binding to the midgut epithelial cells of many lepidopteran larvae.

Product Specifications

Recombinant Bacillus thuringiensis subsp. kurstaki Pesticidal crystal protein cry1Ac (cry1Ac), partial is a recombinant protein from Bacillus thuringiensis subsp. kurstaki, expressed in Yeast, covering amino acids 972-1178aa, with N-terminal 6xHis-tagged tag, molecular weight 25.8kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Promotes colloidosmotic lysis by binding to the midgut epithelial cells of many lepidopteran larvae

Gene: cry1Ac  |  Organism: Bacillus thuringiensis subsp. kurstaki  |  Synonyms: 133 kDa crystal protein; Crystaline entomocidal protoxin; Insecticidal delta-endotoxin CryIA(c)

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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