Mouse anti-Human/Mouse/Rat BCL2 Monoclonal Antibody

Function

Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells (PubMed:1508712, PubMed:8183370). Regulates cell death by controlling the mitochondrial membrane permeability (PubMed:11368354). Appears to function in a feedback loop system with caspases (PubMed:11368354). Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1) (PubMed:11368354). Also acts as an inhibitor of autophagy: interacts with BECN1 and AMBRA1 during non-starvation conditions and inhibits their autophagy function (PubMed:18570871, PubMed:20889974, PubMed:21358617). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed:17418785)

Subcellular Location

Mitochondrion outer membrane (Single-pass membrane protein); Nucleus membrane (Single-pass membrane protein); Endoplasmic reticulum membrane (Single-pass membrane protein); Cytoplasm

Disease Association

[A chromosomal aberration involving BCL2 has been found in chronic lymphatic leukemia. Translocation t(14;18)(q32;q21) with immunoglobulin gene regions. BCL2 mutations found in non-Hodgkin lymphomas carrying the chromosomal translocation could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions]

Tissue Specificity

Expressed in a variety of tissues

Subunit

(Microbial infection) Interacts with Toxoplasma gondii ROP17; the interaction probably promotes BCL2 phosphorylation and degradation

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• PubMed: CI-9GAL9GH7V57OH (JAN-)
• Nusbaum C. et al. DNA sequence and analysis of human chromosome 18. — Nature (2005)
• Mural R.J. et al. PubMed: CI-56FT96O07V0QG (JUL-)