Mouse anti-Human/Mouse/Rat sapiens (Human) ANAPC2 Monoclonal Antibody

Function

Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through the cell cycle (PubMed:11739784, PubMed:18485873, PubMed:27120157, PubMed:27509861). Together with the RING-H2 protein ANAPC11, forms the catalytic component of the E3 ubiquitin ligase complex (PubMed:11739784, PubMed:18485873, PubMed:27120157, PubMed:27509861). APC/C acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains (PubMed:11739784, PubMed:18485873). APC/C catalyzes assembly of branched 'Lys-11'-/'Lys-48'-linked branched ubiquitin chains on target proteins (PubMed:29033132). APC/C is activated by CDC20 in the metaphase/anaphase transition of cell cycle, targeting the degradation of cyclin B and securin (PubMed:27120157, PubMed:27509861). APC/C is regulated by the mitotic checkpoint complex (MCC), which inhibits APC/C and delays chromosome segregation (PubMed:27509861). The CDC20-APC/C complex positively regulates the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons (By similarity). CDC20-APC/C-induced degradation of NEUROD2 drives presynaptic differentiation (By similarity)

Subunit

Component of the anaphase promoting complex/cyclosome (APC/C), composed of ANAPC1, ANAPC2, CDC27/ANAPC3, ANAPC4, ANAPC5, CDC16/ANAPC6, ANAPC7, CDC23/ANAPC8, ANAPC10, ANAPC11, CDC26/ANAPC12, ANAPC13, ANAPC15 and ANAPC16 (PubMed:25043029, PubMed:26083744, PubMed:27120157, PubMed:27509861). APC/C associates with CDC20 to form the CDC20-APC/C complex, which is crucial for metaphase/anaphase transition in cell cycle; this interaction is dependent on APC/C phosphorylation (PubMed:27120157, PubMed:27509861). APC/C associates with mitotic checkpoint complex (MCC), which inhibits APC/C and delays chromosome segregation (PubMed:27509861). APC/C interacts with FZR1 and FBXO5 (PubMed:25043029, PubMed:26083744). APC/C interacts with UBE2C and UBE2S; the interactions are direct (PubMed:27259151). Interacts (via cullin domain) with ANAPC11 and with UBCH10 (PubMed:11739784). Interacts with NEUROD2 (By similarity). Interacts with FBXO43; the interaction is direct (PubMed:34595750)

Pathway

Protein modification; protein ubiquitination

• Yu H. et al. Identification of a cullin homology region in a subunit of the anaphase-promoting complex. — Science (1998)
• Humphray S.J. et al. DNA sequence and analysis of human chromosome 9. — Nature (2004)
• The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). — Genome Res. (2004)
• Nagase T. et al. Prediction of the coding sequences of unidentified human genes. XVI. The complete sequences of 150 new cDNA clones from brain which code for large proteins in vitro. — DNA Res. (2000)
• Nakajima D. et al. Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones. — DNA Res. (2002)
• Tang Z. et al. APC2 cullin protein and APC11 RING protein comprise the minimal ubiquitin ligase module of the anaphase-promoting complex. — Mol. Biol. Cell (2001)
• Kraft C. et al. Mitotic regulation of the human anaphase-promoting complex by phosphorylation. — EMBO J. (2003)
• Beausoleil S.A. et al. A probability-based approach for high-throughput protein phosphorylation analysis and site localization. — Nat. Biotechnol. (2006)