Mouse anti-Human/Mouse/Rat sapiens (Human) ASCC2 Monoclonal Antibody

Function

Ubiquitin-binding protein involved in DNA repair and rescue of stalled ribosomes (PubMed:29144457, PubMed:32099016, PubMed:32579943, PubMed:36302773). Plays a role in DNA damage repair as component of the ASCC complex (PubMed:29144457). Recruits ASCC3 and ALKBH3 to sites of DNA damage by binding to polyubiquitinated proteins that have 'Lys-63'-linked polyubiquitin chains (PubMed:29144457). Part of the ASC-1 complex that enhances NF-kappa-B, SRF and AP1 transactivation (PubMed:12077347). Involved in activation of the ribosome quality control (RQC) pathway, a pathway that degrades nascent peptide chains during problematic translation (PubMed:32099016, PubMed:32579943, PubMed:36302773). Specifically recognizes and binds RPS20/uS10 ubiquitinated by ZNF598, promoting recruitment of the RQT (ribosome quality control trigger) complex on stalled ribosomes, followed by disassembly of stalled ribosomes (PubMed:36302773)

Subcellular Location

Nucleus; Nucleus speckle

Tissue Specificity

Ubiquitous

Subunit

Identified in the ASCC complex that contains ASCC1, ASCC2 and ASCC3 (PubMed:29144457, PubMed:29997253). Interacts directly with ASCC3 (PubMed:29997253, PubMed:32099016). The ASCC complex interacts with ALKBH3 (PubMed:22055184, PubMed:29144457). Interacts (via CUE domain) with 'Lys-63'-linked polyubiquitin chains, but not with 'Lys-48'-linked polyubiquitin chains (PubMed:29144457). Part of the ASC-1 complex, that contains TRIP4, ASCC1, ASCC2 and ASCC3 (PubMed:12077347). Component of the RQT (ribosome quality control trigger) complex, that contains ASCC2, ASCC3 and TRIP4 (PubMed:32099016, PubMed:32579943, PubMed:36302773). Interacts with CSRP1 (PubMed:26924529). Interacts with PRPF8, a component of the spliceosome (PubMed:29144457). Interacts with ZCCHC4 (PubMed:31799605)

• Jung D.-J. et al. Novel transcription coactivator complex containing activating signal cointegrator 1. — Mol. Cell. Biol. (2002)
• Ota T. et al. Complete sequencing and characterization of 21,243 full-length human cDNAs. — Nat. Genet. (2004)
• Dunham I. et al. The DNA sequence of human chromosome 22. — Nature (1999)
• The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). — Genome Res. (2004)
• Yu L.R. et al. Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra. — J. Proteome Res. (2007)
• Olsen J.V. et al. Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis. — Sci. Signal. (2010)
• Burkard T.R. et al. Initial characterization of the human central proteome. — BMC Syst. Biol. (2011)
• Dango S. et al. DNA unwinding by ASCC3 helicase is coupled to ALKBH3-dependent DNA alkylation repair and cancer cell proliferation. — Mol. Cell (2011)