Mouse anti-Human/Mouse/Rat sapiens (Human) DDX50 Monoclonal Antibody
Mouse anti-Human/Mouse/Rat sapiens (Human) DDX50 Monoclonal Antibody - Image 2

Mouse anti-Human/Mouse/Rat sapiens (Human) DDX50 Monoclonal Antibody

Mouse anti-Human/Mouse/Rat sapiens (Human) DDX50 Monoclonal Antibody — ATP-dependent RNA helicase that may play a role in various aspects of RNA metabolism including pre-mRNA splicing or ribosomal RNA production.

SKU: BCREC-000260MA Category:
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Product Specifications

Uniprot No.Q9BQ39
Target NamesDDX50
Species ReactivityMouse
ImmunogenRecombinant Human DDX50 protein
Immunogen SpeciesHomo sapiens (Human)
ConjugateNon-conjugated
IsotypeMouse IgG2b
ClonalityMonoclonal
ApplicationsELISA, WB, ICC
BufferPBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
StorageUpon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Function

ATP-dependent RNA helicase that may play a role in various aspects of RNA metabolism including pre-mRNA splicing or ribosomal RNA production. Also acts as a viral restriction factor and promotes the activation of the NF-kappa-B and IRF3 signaling pathways following its stimulation with viral RNA or infection with RNA and DNA viruses. For instance, decreases vaccinia virus, herpes simplex virus, Zika virus or dengue virus replication during the early stage of infection.

Biological Context

Subcellular Location: Nucleus, nucleolus; Cytoplasm
Tissue Specificity: Highest expression in skeletal muscle, liver, heart, placenta, and kidney

Product Specifications

Mouse anti-Human/Mouse/Rat sapiens (Human) DDX50 Monoclonal Antibody is a recombinant protein. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

ATP-dependent RNA helicase that may play a role in various aspects of RNA metabolism including pre-mRNA splicing or ribosomal RNA production (PubMed:12027455). Also acts as a viral restriction factor and promotes the activation of the NF-kappa-B and IRF3 signaling pathways following its stimulation with viral RNA or infection with RNA and DNA viruses (PubMed:35215908). For instance, decreases vaccinia virus, herpes simplex virus, Zika virus or dengue virus replication during the early stage of infection (PubMed:28181036, PubMed:35215908). Mechanistically, acts via the adapter TICAM1 and independently of the DDX1-DDX21-DHX36 helicase complex to induce the production of interferon-beta (PubMed:35215908)

Subcellular Location

Nucleus, nucleolus; Cytoplasm

Tissue Specificity

Highest expression in skeletal muscle, liver, heart, placenta, and kidney

Subunit

Interacts with C1QBP. Interacts with the ubiquitin ligase CTLH complex through GID4 (PubMed:38773330). Interacts with TICAM1 (PubMed:35215908)

Gene: DDX50  |  Organism: Homo sapiens  |  Synonyms: DEAD box protein 50; Gu-beta; Nucleolar protein Gu2
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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