Mouse anti-Human/Mouse/Rat sapiens (Human) NEDD9 Monoclonal Antibody

Function

Scaffolding protein which plays a central coordinating role for tyrosine-kinase-based signaling related to cell adhesion (PubMed:24574519). As a focal adhesion protein, plays a role in embryonic fibroblast migration (By similarity). May play an important role in integrin beta-1 or B cell antigen receptor (BCR) mediated signaling in B- and T-cells. Integrin beta-1 stimulation leads to recruitment of various proteins including CRKL and SHPTP2 to the tyrosine phosphorylated form (PubMed:9020138). Promotes adhesion and migration of lymphocytes; as a result required for the correct migration of lymphocytes to the spleen and other secondary lymphoid organs (PubMed:17174122). Plays a role in the organization of T-cell F-actin cortical cytoskeleton and the centralization of T-cell receptor microclusters at the immunological synapse (By similarity). Negatively regulates cilia outgrowth in polarized cysts (By similarity). Modulates cilia disassembly via activation of AURKA-mediated phosphorylation of HDAC6 and subsequent deacetylation of alpha-tubulin (PubMed:17604723). Positively regulates RANKL-induced osteoclastogenesis (By similarity). Required for the maintenance of hippocampal dendritic spines in the dentate gyrus and CA1 regions, thereby involved in spatial learning and memory (By similarity)

Subcellular Location

Cytoplasm, cytoskeleton, spindle

Tissue Specificity

Expressed in B-cells (at protein level) (PubMed:9020138). Expressed in the respiratory epithelium of the main bronchi to the bronchioles in the lungs (at protein level) (PubMed:9584194). High levels detected in kidney, lung, and placenta (PubMed:9584194). Expressed in lymphocytes (PubMed:9497377)

Subunit

Homodimer (PubMed:10502414, PubMed:9584194). Forms heterodimers with BCAR1/p130cas (PubMed:10502414). Forms complexes with PTK2B/RAFTK, adapter protein CRKL and LYN kinase (PubMed:9020138). Part of a complex composed of NEDD9, AURKA and CTTN; within the complex NEDD9 acts as a scaffold protein and is required for complex formation (PubMed:24574519). Part of a ternary complex composed of SMAD3, ITCH/AIP4 and NEDD9/HEF1; within the complex NEDD9/HEF1 interacts (via N-terminus) with ITCH/AIP4 (via WW domains); the complex mediates ubiquitination and proteasomal degradation of NEDD9/HEF1 (PubMed:15051726). Interacts with SMAD3; the interaction promotes NEDD9 ubiquitination and proteasomal degradation (PubMed:24574519). Interacts with ID2 (PubMed:10502414). Interacts with CTTN (via N-terminus) (PubMed:24574519). Interacts with MICAL (PubMed:11827972). Interacts with TXNL4/DIM1 (PubMed:11054566). Interacts with BCAR3 (via Ras-GEF domain) (PubMed:19103205). Interacts with SH2D3C isoform 1 and isoform 2 (By similarity). Interacts with ECT2 (PubMed:16394104). Interacts with PTPN11/SHP-2 (via SH2 domains); the interaction is enhanced when NEDD9/CAS-L is tyrosine phosphorylated (PubMed:19275884). Interacts (via C-terminus) with PLK1 (via polo box domains) (PubMed:29191835). Interacts with NKX2-5 (PubMed:29899023). Interacts with SMAD3; the interaction is inhibited by oxidation of NEDD9 (PubMed:15051726, PubMed:29899023). Interacts with NEDD9/HEF1; interaction is induced by CXCL12 promotion of ABL-mediated phosphorylation of NEDD9/HEF1 (PubMed:22810897). Interacts (via SH3 domain) with PTK2/FAK (PubMed:8668148, PubMed:8879209, PubMed:9360983). Interacts with FYN; in the presence of PTK2 (PubMed:9360983). Interacts with INPPL1/SHIP2 (By similarity)

• Law S.F. et al. Human enhancer of filamentation 1, a novel p130cas-like docking protein, associates with focal adhesion kinase and induces pseudohyphal growth in Saccharomyces cerevisiae. — Mol. Cell. Biol. (1996)
• Minegishi M. et al. Structure and function of Cas-L, a 105-kD Crk-associated substrate-related protein that is involved in beta 1 integrin-mediated signaling in lymphocytes. — J. Exp. Med. (1996)
• Ota T. et al. Complete sequencing and characterization of 21,243 full-length human cDNAs. — Nat. Genet. (2004)
• Mungall A.J. et al. The DNA sequence and analysis of human chromosome 6. — Nature (2003)
• Mural R.J. et al. PubMed: CI-5GBDQ6B103N1E (JUL-)
• The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). — Genome Res. (2004)
• Manie S.N. et al. Involvement of p130(Cas) and p105(HEF1), a novel Cas-like docking protein, in a cytoskeleton-dependent signaling pathway initiated by ligation of integrin or antigen receptor on human B cells. — J. Biol. Chem. (1997)
• Tachibana K. et al. Tyrosine phosphorylation of Crk-associated substrates by focal adhesion kinase. A putative mechanism for the integrin-mediated tyrosine phosphorylation of Crk-associated substrates. — J. Biol. Chem. (1997)