Mouse anti-Human/Mouse/Rat sapiens (Human) ROS1 Monoclonal Antibody

Function

Receptor tyrosine kinase (RTK) that plays a role in epithelial cell differentiation and regionalization of the proximal epididymal epithelium. NELL2 is an endogenous ligand for ROS1. Upon endogenous stimulation by NELL2, ROS1 activates the intracellular signaling pathway and triggers epididymal epithelial differentiation and subsequent sperm maturation (By similarity). May activate several downstream signaling pathways related to cell differentiation, proliferation, growth and survival including the PI3 kinase-mTOR signaling pathway. Mediates the phosphorylation of PTPN11, an activator of this pathway. May also phosphorylate and activate the transcription factor STAT3 to control anchorage-independent cell growth. Mediates the phosphorylation and the activation of VAV3, a guanine nucleotide exchange factor regulating cell morphology. May activate other downstream signaling proteins including AKT1, MAPK1, MAPK3, IRS1 and PLCG2

Subcellular Location

Cell membrane (Single-pass type I membrane protein)

Disease Association

[A chromosomal aberration involving ROS1 is found in a glioblastoma multiforme sample. An intra-chromosomal deletion del(6)(q21q21) is responsible for the formation of GOPC-ROS1 chimeric protein which is localized to the Golgi and has a constitutive receptor tyrosine kinase activity. A SLC34A2-ROS1 chimeric protein produced in non-small cell lung cancer cells also retains a constitutive kinase activity. A third type of chimeric protein CD74-ROS1 was also identified in those cells]

Tissue Specificity

Expressed in brain. Expression is increased in primary gliomas

Subunit

Interacts with PTPN6 (via SH2 1 domain); the interaction is direct and promotes ROS1 dephosphorylation (By similarity). Interacts with PTPN11; may activate the PI3 kinase-mTOR signaling pathway (PubMed:16885344). Interacts with VAV3; constitutive interaction mediating VAV3 phosphorylation (PubMed:11094073)

• Birchmeier C. et al. Characterization of ROS1 cDNA from a human glioblastoma cell line. — Proc. Natl. Acad. Sci. U.S.A. (1990)
• Mungall A.J. et al. The DNA sequence and analysis of human chromosome 6. — Nature (2003)
• Matsushime H. et al. Human c-ros-1 gene homologous to the v-ros sequence of UR2 sarcoma virus encodes for a transmembrane receptorlike molecule. — Mol. Cell. Biol. (1986)
• Birchmeier C. et al. Characterization of an activated human ros gene. — Mol. Cell. Biol. (1986)
• Watkins D. et al. Analysis of oncogene expression in primary human gliomas: evidence for increased expression of the ros oncogene. — Cancer Genet. Cytogenet. (1994)
• Zeng L. et al. Vav3 mediates receptor protein tyrosine kinase signaling, regulates GTPase activity, modulates cell morphology, and induces cell transformation. — Mol. Cell. Biol. (2000)
• Charest A. et al. Fusion of FIG to the receptor tyrosine kinase ROS in a glioblastoma with an interstitial del(6)(q21q21). — Genes Chromosomes Cancer (2003)
• Charest A. et al. Oncogenic targeting of an activated tyrosine kinase to the Golgi apparatus in a glioblastoma. — Proc. Natl. Acad. Sci. U.S.A. (2003)