Mouse anti-Human/Rabbit sapiens (Human) CD63 Monoclonal Antibody

Function

Functions as a cell surface receptor for TIMP1 and plays a role in the activation of cellular signaling cascades. Plays a role in the activation of ITGB1 and integrin signaling, leading to the activation of AKT, FAK/PTK2 and MAP kinases. Promotes cell survival, reorganization of the actin cytoskeleton, cell adhesion, spreading and migration, via its role in the activation of AKT and FAK/PTK2. Plays a role in VEGFA signaling via its role in regulating the internalization of KDR/VEGFR2. Plays a role in intracellular vesicular transport processes, and is required for normal trafficking of the PMEL luminal domain that is essential for the development and maturation of melanocytes. Plays a role in the adhesion of leukocytes onto endothelial cells via its role in the regulation of SELP trafficking. May play a role in mast cell degranulation in response to Ms4a2/FceRI stimulation, but not in mast cell degranulation in response to other stimuli

Subcellular Location

Cell membrane (Multi-pass membrane protein); Lysosome membrane (Multi-pass membrane protein); Late endosome membrane (Multi-pass membrane protein); Endosome, multivesicular body; Melanosome; Secreted, extracellular exosome; Cell surface

Tissue Specificity

Detected in platelets (at protein level). Dysplastic nevi, radial growth phase primary melanomas, hematopoietic cells, tissue macrophages

Subunit

Interacts with TIMP1 and ITGB1 and recruits TIMP1 to ITGB1 (PubMed:16917503, PubMed:24635319). Interacts with CD9. Identified in a complex with CD9 and ITGB3 (PubMed:19640571). Interacts with PMEL (PubMed:21962903). Interacts with KDR/VEGFR2; identified in a complex with ITGB1 and KDR/VEGFR2 and is required to recruit KDR to ITGB1 complexes (PubMed:23632027). Interacts with SYT7 (By similarity)

• Hotta H. et al. Molecular cloning and characterization of an antigen associated with early stages of melanoma tumor progression. — Cancer Res. (1988)
• Rapp G. et al. Characterization of three abundant mRNAs from human ovarian granulosa cells. — DNA Cell Biol. (1990)
• Metzelaar M.J. et al. CD63 antigen. A novel lysosomal membrane glycoprotein, cloned by a screening procedure for intracellular antigens in eukaryotic cells. — J. Biol. Chem. (1991)
• Wang M.X. et al. An ocular melanoma-associated antigen. Molecular characterization. — Arch. Ophthalmol. (1992)
• Hotta H. et al. Genomic structure of the ME491/CD63 antigen gene and functional analysis of the 5'-flanking regulatory sequences. — Biochem. Biophys. Res. Commun. (1992)
• Ancans J. et al. Melanocyte variant of lysosome-associated membrane protein-3 (LAMP3; also CD63 and melanoma associated antigen ME419) mRNA. (MAY-)
• Ota T. et al. Complete sequencing and characterization of 21,243 full-length human cDNAs. — Nat. Genet. (2004)
• Halleck A. et al. Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201). (JUN-)