Mouse anti-Human sapiens (Human) CEP55 Monoclonal Antibody

Function

Plays a role in mitotic exit and cytokinesis (PubMed:16198290, PubMed:17853893). Recruits PDCD6IP and TSG101 to midbody during cytokinesis. Required for successful completion of cytokinesis (PubMed:17853893). Not required for microtubule nucleation (PubMed:16198290). Plays a role in the development of the brain and kidney (PubMed:28264986)

Subcellular Location

Cytoplasm; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome; Cleavage furrow; Midbody, Midbody ring; Midbody

Disease Association

Multinucleated neurons, anhydramnios, renal dysplasia, cerebellar hypoplasia and hydranencephaly (MARCH) : An autosomal recessive, congenital disease characterized by severe hydranencephaly with multinucleated neurons, renal aplasia or dysplasia, and hypoplastic kidneys. Hydranencephaly is an anomaly leading to replacement of the cerebral hemispheres with a fluid-filled cyst. MARCH results in death in utero or in the perinatal period. [The disease is caused by variants affecting the gene represented in this entry]

Tissue Specificity

Expressed in embryonic brain (PubMed:28264986). Expressed in fetal brain ganglionic eminence, kidney tubules and multinucleate neurons in the temporal cortex (PubMed:28264986). Expressed in adult brain, cerebellum, kidney tubules, intestine and muscles (at protein level) (PubMed:28264986, PubMed:28295209). Widely expressed, mostly in proliferative tissues. Highly expressed in testis. Intermediate levels in adult and fetal thymus, as well as in various cancer cell lines. Low levels in different parts of the digestive tract, bone marrow, lymph nodes, placenta, fetal heart and fetal spleen. Hardly detected in brain

Subunit

Homodimer (PubMed:16406728). Interacts (phosphorylated on Ser-425 and Ser-428) with PLK1; the interaction is indirect via the MTMR3:MTMR4 heterooligomer, occurs during early mitosis, regulates the phosphorylation of CEP55 by PLK1 and its recruitment to the midbody where it can mediate cell abscission (PubMed:16198290, PubMed:25659891). Interacts with AKAP9/CG-NAP; the interaction occurs in interphase and is lost upon mitotic entry (PubMed:16198290). Interacts with PCNT/Kendrin; the interaction occurs in interphase and is lost upon mitotic entry (PubMed:16198290). Directly interacts with PDCD6IP; this interaction is required for PDCD6IP targeting to the midbody; CEP55 binds PDCD6IP in a 2:1 stoichiometry; PDCD6IP competes with TSG101 for the same binding site (PubMed:17556548, PubMed:17853893, PubMed:18641129, PubMed:18948538). Interacts with TSG101; TSG101 competes with PDCD6IP for the same binding site; interaction is required for cytokinesis but not for viral budding (PubMed:17556548, PubMed:17853893, PubMed:18948538). Interacts with MVB12A, VPS37B, VPS37C and VPS28 (PubMed:17853893)

• Martinez-Garay I. et al. The novel centrosomal associated protein CEP55 is present in the spindle midzone and the midbody. — Genomics (2006)
• Shimokawa T. et al. Cloning and characterization of URCC6, a novel gene up-regulated in colon cancer. (SEP-)
• Ota T. et al. Complete sequencing and characterization of 21,243 full-length human cDNAs. — Nat. Genet. (2004)
• Totoki Y. et al. PubMed: CI-58UKVFP8TE70J (APR-)
• Bechtel S. et al. The full-ORF clone resource of the German cDNA consortium. — BMC Genomics (2007)
• Deloukas P. et al. The DNA sequence and comparative analysis of human chromosome 10. — Nature (2004)
• Mural R.J. et al. PubMed: CI-5GBD0VIIJ7C63 (SEP-)
• The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). — Genome Res. (2004)