Mouse anti-Human sapiens (Human) CKB Monoclonal Antibody

Function

Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate) (PubMed:8186255). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa (Probable). Acts as a key regulator of adaptive thermogenesis as part of the futile creatine cycle: localizes to the mitochondria of thermogenic fat cells and acts by mediating phosphorylation of creatine to initiate a futile cycle of creatine phosphorylation and dephosphorylation (By similarity). During the futile creatine cycle, creatine and N-phosphocreatine are in a futile cycle, which dissipates the high energy charge of N-phosphocreatine as heat without performing any mechanical or chemical work (By similarity)

Subcellular Location

Cytoplasm, cytosol; Mitochondrion; Cell membrane

Subunit

Dimer of identical or non-identical chains, which can be either B (brain type) or M (muscle type). With MM being the major form in skeletal muscle and myocardium, MB existing in myocardium, and BB existing in many tissues, especially brain. Interacts with SLC12A6 (via C-terminus); the interaction may be required for SLC12A6 potassium-chloride cotransport activity (PubMed:18566107)

• Villarreal-Levy G. et al. Human creatine kinase: isolation and sequence analysis of cDNA clones for the B subunit, development of subunit specific probes and determination of gene copy number. — Biochem. Biophys. Res. Commun. (1987)
• Mariman E.C.M. et al. Structure and expression of the human creatine kinase B gene. — Genomics (1987)
• Kaye F.J. et al. Human creatine kinase-B complementary DNA. Nucleotide sequence, gene expression in lung cancer, and chromosomal assignment to two distinct loci. — J. Clin. Invest. (1987)
• Mariman E.C.M. et al. Complete nucleotide sequence of the human creatine kinase B gene. — Nucleic Acids Res. (1989)
• Ota T. et al. Complete sequencing and characterization of 21,243 full-length human cDNAs. — Nat. Genet. (2004)
• Halleck A. et al. Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201). (JUN-)
• PubMed: CI-7CAL73TV6F1SO (DEC-)
• Mural R.J. et al. PubMed: CI-5GBDQ6B103N1E (JUL-)