Mouse anti-Human sapiens (Human) KRT6A Monoclonal Antibody

Function

Structural component of intermediate filaments in epithelial keratinocytes. Forms heteropolymers with the type I keratins KRT16 and KRT17, assembling into keratin intermediate filament networks that contributes to the structural integrity and stress resilience of stratified epithelia, particularly in epidermis, nail bed and oral mucosa (PubMed:11886499, PubMed:17719747, PubMed:7545493). Rapidly induced in wound-edge keratinocytes and participates in cytoskeletal reorganization during re-epithelialization (By similarity). Negatively regulates collective keratinocyte migration by stabilizing non-muscle myosin MYH9 and desmoplakin, thereby altering cell-cell and cell-matrix adhesion and reducing the speed and directionality of epithelial sheet movement during wound repair (By similarity). Also limits epithelial migration by inhibiting SRC activity during wound repair (By similarity). Required for normal palmoplantar, nail unit and oral mucosa integrity (PubMed:11886499, PubMed:17719747, PubMed:7545493)

Subcellular Location

Cytoplasm

Disease Association

Pachyonychia congenita 3 (PC3) : An autosomal dominant genodermatosis characterized by hypertrophic nail dystrophy, painful and highly debilitating plantar keratoderma, oral leukokeratosis, and a variety of epidermal cysts. [The disease is caused by variants affecting the gene represented in this entry]

Tissue Specificity

Expressed in the corneal epithelium (at protein level)

Subunit

Heterodimers composed of one type I and one type II keratins; forms parallel coiled-coil heterodimers (By similarity). Heterodimers associate in an antiparallel manner to form heterotetramers, which further assemble into higher-order keratin intermediate filaments (By similarity). Associates with KRT16 and/or KRT17 (By similarity). Interacts with TCHP (PubMed:15731013). Interacts with IIA/MYH2; the interaction stabilizes MYH2 (By similarity)

• Takahashi K. et al. Cloning and characterization of multiple human genes and cDNAs encoding highly related type II keratin 6 isoforms. — J. Biol. Chem. (1995)
• Kalnine N. et al. Cloning of human full-length CDSs in BD Creator(TM) system donor vector. (MAY-)
• The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). — Genome Res. (2004)
• Bienvenut W.V. et al. PubMed: CI-F4M0Q0JINDU1V (MAR-)
• Hanukoglu I. et al. The cDNA sequence of a type II cytoskeletal keratin reveals constant and variable structural domains among keratins. — Cell (1983)
• Nishizawa M. et al. Identification of trichoplein, a novel keratin filament-binding protein. — J. Cell Sci. (2005)
• Burkard T.R. et al. Initial characterization of the human central proteome. — BMC Syst. Biol. (2011)
• Du Z.F. et al. Two novel de novo mutations of KRT6A and KRT16 genes in two Chinese pachyonychia congenita pedigrees with fissured tongue or diffuse plantar keratoderma. — Eur. J. Dermatol. (2012)