Mouse anti-Human sapiens (Human) MCM9 Monoclonal Antibody

Function

Component of the MCM8-MCM9 complex, which is involved in the repair of double-stranded DNA breaks (DBSs) and DNA interstrand cross-links (ICLs) by homologous recombination (HR) (PubMed:23401855). The MCM8-MCM9 complex is a 3'-5' DNA helicase and single-stranded (ss)DNA-stimulated ATPase which binds ssDNA in the presence of nucleoside triphosphates (PubMed:37309874). Required for DNA resection by the MRE11-RAD50-NBN/NBS1 (MRN) complex by recruiting the MRN complex to the repair site and by promoting the complex nuclease activity (PubMed:26215093). Indirectly regulates the recruitment of downstream effector RAD51 to DNA damage sites including DBSs and ICLs, probably by regulating the localization of the MNR complex (PubMed:23401855). Acts as a helicase in DNA mismatch repair (MMR) following DNA replication errors to unwind the mismatch containing DNA strand (PubMed:26300262). In addition, recruits MLH1, a component of the MMR complex, to chromatin (PubMed:26300262). The MCM8-MCM9 complex is dispensable for DNA replication and S phase progression (PubMed:23401855). Plays a key role during gametogenesis, probably by regulating HR (By similarity)

Subcellular Location

Nucleus; Chromosome

Disease Association

Ovarian dysgenesis 4 (ODG4) : A form of ovarian dysgenesis, a disorder characterized by lack of spontaneous pubertal development, primary amenorrhea, uterine hypoplasia, and hypergonadotropic hypogonadism as a result of streak gonads. ODG4 is an autosomal recessive condition. [The disease is caused by variants affecting the gene represented in this entry]

Subunit

Component of the MCM8-MCM9 complex, which forms a heterohexamer (PubMed:23401855, PubMed:26215093, PubMed:26300262, PubMed:34043945, PubMed:37309874) with a repeating heterodimer of trimers configuration (PubMed:34043945, PubMed:37309874). Interacts with the DNA mismatch repair (MMR) complex composed at least of MSH2, MSH3, MSH6, PMS1 and MLH1 (PubMed:26300262). Interacts with MLH1; the interaction recruits MLH1 to chromatin (PubMed:26300262). Interacts with MSH2; the interaction recruits MCM9 to chromatin (PubMed:26300262). Interacts with MSH6 (PubMed:26300262). Interacts with the MRN complex composed of MRE11, RAD50 and NBN/NBS1; the interaction recruits the MRN complex to DNA damage sites (PubMed:26215093). Interacts with RAD51; the interaction recruits RAD51 to DNA damage sites (PubMed:23401855). Interacts with HROB; the interaction recruits the MCM8-MCM9 complex to DNA damage sites (PubMed:31467087) and stimulates the helicase activity (PubMed:37535404)

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• Mungall A.J. et al. The DNA sequence and analysis of human chromosome 6. — Nature (2003)
• The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). — Genome Res. (2004)
• Lutzmann M. et al. Identification of full genes and proteins of MCM9, a novel, vertebrate-specific member of the MCM2-8 protein family. — Gene (2005)
• Dephoure N. et al. A quantitative atlas of mitotic phosphorylation. — Proc. Natl. Acad. Sci. U.S.A. (2008)
• Olsen J.V. et al. Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis. — Sci. Signal. (2010)
• Jeffries E.P. et al. Identification, quantification, and evolutionary analysis of a novel isoform of MCM9. — Gene (2013)
• Zhou H. et al. Toward a comprehensive characterization of a human cancer cell phosphoproteome. — J. Proteome Res. (2013)