Mouse anti-Human sapiens (Human) SNX9 Monoclonal Antibody

Function

Involved in endocytosis and intracellular vesicle trafficking, both during interphase and at the end of mitosis. Required for efficient progress through mitosis and cytokinesis. Required for normal formation of the cleavage furrow at the end of mitosis. Plays a role in endocytosis via clathrin-coated pits, but also clathrin-independent, actin-dependent fluid-phase endocytosis. Plays a role in macropinocytosis. Promotes internalization of TNFR. Promotes degradation of EGFR after EGF signaling. Stimulates the GTPase activity of DNM1. Promotes DNM1 oligomerization. Promotes activation of the Arp2/3 complex by WASL, and thereby plays a role in the reorganization of the F-actin cytoskeleton. Binds to membranes enriched in phosphatidylinositol 4,5-bisphosphate and promotes membrane tubulation. Has lower affinity for membranes enriched in phosphatidylinositol 3-phosphate

Subcellular Location

Cytoplasmic vesicle membrane (Peripheral membrane protein); Cell membrane (Peripheral membrane protein); Cytoplasmic vesicle, clathrin-coated vesicle; Golgi apparatus, trans-Golgi network; Cell projection, ruffle; Cytoplasm

Tissue Specificity

Widely expressed, with highest levels in heart and placenta, and lowest levels in thymus and peripheral blood leukocytes

Subunit

Homodimer, and homooligomer. Heterodimer with SNX18. Interacts with ITCH. Interacts (via SH3 domain) with TNK2, WASL and ACTR3. Identified in a complex with TNK2 and clathrin heavy chains. Identified in a complex with the AP-2 complex, clathrin and DNM2. Interacts (via SH3 domain) with DNM1 and DNM2. Identified in an oligomeric complex containing DNM1 and SNX9. Interacts with FCHSD1 (By similarity). Interacts with ADAM9 and ADAM15 cytoplasmic tails

• Teasdale R.D. et al. A large family of endosome-localized proteins related to sorting nexin 1. — Biochem. J. (2001)
• Howard L. et al. Interaction of the metalloprotease disintegrins MDC9 and MDC15 with two SH3 domain-containing proteins, endophilin I and SH3PX1. — J. Biol. Chem. (1999)
• Zhang J.S. et al. Identification of differentially expressed genes in matched prostate cancer and normal epithelial cell lines. (JUL-)
• Mungall A.J. et al. The DNA sequence and analysis of human chromosome 6. — Nature (2003)
• The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). — Genome Res. (2004)
• Ramanathan G. et al. Human SDP1. (JUL-)
• Lin Q. et al. The Cdc42 target ACK2 interacts with sorting nexin 9 (SH3PX1) to regulate epidermal growth factor receptor degradation. — J. Biol. Chem. (2002)
• Lundmark R. et al. Sorting nexin 9 participates in clathrin-mediated endocytosis through interactions with the core components. — J. Biol. Chem. (2003)