Mouse anti-Human sapiens (Human) SP1 Monoclonal Antibody

Function

Transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Also binds the PDGFR-alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling. Plays an essential role in the regulation of FE65 gene expression. In complex with ATF7IP, maintains telomerase activity in cancer cells by inducing TERT and TERC gene expression. Isoform 3 is a stronger activator of transcription than isoform 1. Positively regulates the transcription of the core clock component BMAL1 (PubMed:10391891, PubMed:11371615, PubMed:11904305, PubMed:14593115, PubMed:16377629, PubMed:16478997, PubMed:16943418, PubMed:17049555, PubMed:18171990, PubMed:18199680, PubMed:18239466, PubMed:18513490, PubMed:18619531, PubMed:19193796, PubMed:20091743, PubMed:21046154, PubMed:21798247). Plays a role in the recruitment of SMARCA4/BRG1 on the c-FOS promoter. Plays a role in protecting cells against oxidative stress following brain injury by regulating the expression of RNF112 (By similarity)

Subcellular Location

Nucleus; Cytoplasm

Tissue Specificity

Up-regulated in adenocarcinomas of the stomach (at protein level). Isoform 3 is ubiquitously expressed at low levels

Subunit

(Microbial infection) Interacts with HIV-1 Vpr; the interaction is inhibited by SP1 O-glycosylation

• Infantino V. et al. Identification of a novel Sp1 splice variant as a strong transcriptional activator. — Biochem. Biophys. Res. Commun. (2011)
• Scherer S.E. et al. The finished DNA sequence of human chromosome 12. — Nature (2006)
• Mural R.J. et al. PubMed: CI-5GBDQ6B103N1E (JUL-)
• The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). — Genome Res. (2004)
• Haggart M.H. et al. PubMed: CI-6VDDHRE0PECGT (APR-)
• Takahara T. et al. Heterogeneous Sp1 mRNAs in human HepG2 cells include a product of homotypic trans-splicing. — J. Biol. Chem. (2000)
• Kadonaga J.T. et al. Isolation of cDNA encoding transcription factor Sp1 and functional analysis of the DNA binding domain. — Cell (1987)
• Nicolas M. et al. Expression of transcription factor Sp1 mRNA in mammalian cells. (APR-)