Newcastle disease virus RNA-directed RNA polymerase L (L), partial Recombinant Protein, C-His

SKU: BCACP-766946 Category:
Product SKUBCACP-766946
Product DescriptionNewcastle disease virus RNA-directed RNA polymerase L (L), partial Recombinant Protein is expressed from E.coli with C-terminal 6xHis-tagged. It contains 1741-1960aa. [Accession | Q9DLD3].
Uniprot No.Q9DLD3
Gene NamesL
Protein LengthPartial
Purity≥ 95% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1741-1960aa
SpeciesNewcastle disease virus (strain Chicken/United States/B1/48) (NDV)
Tag InfoC-terminal 6xHis-tagged
Molecular weight31.6 kDa
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesProtein L;Large structural protein;Replicase;Transcriptase;EC 2.7.7.48;EC 3.6.1.-;EC 2.7.7.88;PRNTase;EC 2.1.1.375;mRNA guanine-N7;G-N7-MTase', "mRNA nucleoside-2'-O-", "N1-2'-O-MTase"]
Protein FamiliesParamyxovirus L protein family

Newcastle disease virus RNA-directed RNA polymerase L (L), partial Recombinant Protein is expressed from E.coli with C-terminal 6xHis-tagged. It contains 1741-1960aa. [Accession | Q9DLD3].

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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